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Amicon® Ultra-15 Centrifugal Filter Units

Provide both ultra-fast spin times and ultra-high recoveries

Overview

Specifications

Ordering Information

Amicon® Ultra-15 Centrifugal Filter UnitsClear Sorting & Filtering Show Filter
Catalog NumberDescriptionNMWLPack Size
UFC900308Amicon® Ultra-15 Centrifugal Filter Unit 3 KDa 8 Show Pricing & Availability
UFC900324Amicon Ultra-15 Centrifugal Filter Unit 3 KDa 24 Show Pricing & Availability
UFC900396Amicon® Ultra-15 Centrifugal Filter Unit 3 KDa 96 Show Pricing & Availability
UFC901008Amicon® Ultra-15 Centrifugal Filter Unit 10 KDa 8 Show Pricing & Availability
UFC901024Amicon® Ultra-15 Centrifugal Filter Unit 10 KDa 24 Show Pricing & Availability
UFC901096Amicon® Ultra-15 Centrifugal Filter Unit 10 KDa 96 Show Pricing & Availability
UFC903008Amicon® Ultra-15 Centrifugal Filter Unit 30 KDa 8 Show Pricing & Availability
UFC903024Amicon® Ultra-15 Centrifugal Filter Unit 30 KDa 24 Show Pricing & Availability
UFC903096Amicon® Ultra-15 Centrifugal Filter Unit 30 KDa 96 Show Pricing & Availability
UFC905008Amicon® Ultra-15 Centrifugal Filter Unit 50kDa 8 Show Pricing & Availability
UFC905024Amicon Ultra-15 Centrifugal Filter Unit 50 KDa 24 Show Pricing & Availability
UFC905096Amicon® Ultra-15 Centrifugal Filter Unit 50 KDa 96 Show Pricing & Availability
UFC910008Amicon Ultra-15 Centrifugal Filter Unit 100 KDa 8 Show Pricing & Availability
UFC910024Amicon® Ultra-15 Centrifugal Filter Unit 100 KDa 24 Show Pricing & Availability
UFC910096Amicon® Ultra-15 Centrifugal Filter Unit 100 KDa 96 Show Pricing & Availability

Amicon® Ultra-15 Filter Units are assembled with centrifuge tubes and caps

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Amicon® Ultra-15 Centrifugal Filter Units, registered for IVD useClear Sorting & Filtering Show Filter
Catalog NumberDescriptionNMWLPack Size
UFC901008DAmicon® Ultra-15 Centrifugal Filter Unit 10 KDa 8 Show Pricing & Availability
UFC901024DAmicon® Ultra-15 Centrifugal Filter Unit 10 KDa 24 Show Pricing & Availability
UFC901096DAmicon® Ultra-15 Centrifugal Filter Unit 10 KDa 96 Show Pricing & Availability

The Amicon® Ultra-15 with Ultracel®-10 membrane with catalog numbers ending in "D" are CE marked and IVD-registered.”

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Documentation

References

Reference overviewApplication
Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis.
Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E.
J Proteome Res. 2006 Apr;5(4):983-7.  2006

Protein Concentration
Ultrafiltration for proteomic sample preparation
E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard
Electrophoresis, 25: 2461-2468  2004

Sample Prep

FAQ

QuestionAnswer
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concentrators?We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.

User Guides

Title
Amicon® Ultra-15 Centrifugal Filter Devices User Guide