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110630 AFB-Color malachite green (oxalate) solution

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      500 ml
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      Catalogue Number110630
      DescriptionAFB-Color malachite green (oxalate) solution
      OverviewAFB-Color malachite green (oxalate) solution - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin., for example smears of enriched bacterial cultures. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. In this case, a modification of the AFB-Color carbol fuchsin ( 108512) solution renders heating unnecessary (Kinyoun method, cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
      Pretreatment of the specimens with Sputofluol® (Cat. No. 108000) dissolves the bacteria from the surrounding viscid sputum and cell material.
      The 500 ml bottle of AFB-Color malachite green (oxalate) solution provides 250 stainings. It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      Product Information
      HS Code3212 90 00
      Quality LevelMQ400
      Applicationfor the microscopic investigation of acid-fast bacteria (AFB) (cold staining)
      Biological Information
      Physicochemical Information
      Density1.0 g/cm3 (20 °C)
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Storage class10 - 13 Other liquids and solids
      WGKWGK 2 obviously hazardous to water
      Aqueous solutions: Container D.
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Suitability for microscopypasses test
      Acid-fast bacteriared
      Backgroundlight green