194434 ABTS™ Chromophore

194434
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Overview

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Key Specifications Table

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194434-100ML
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      Glass bottle 100 ml
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      Description
      OverviewPeroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.
      Catalogue Number194434
      Brand Family Calbiochem®
      Synonyms2,2ʹ-Azino-bis(3-ethylbenzthiazoline-6-sulfonic Acid)
      References
      ReferencesRodgriquez-Lopez, J.N., et al. 2000. Biochemistry 39, 13201.
      Product Information
      CAS number28752-68-3
      DeclarationABTS is a trademark of Boehringer Mannheim Corp.
      FormClear to very pale yellow solution
      FormulationContains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
      Hill FormulaC₁₈H₁₈N₄O₆S₄
      Chemical formulaC₁₈H₁₈N₄O₆S₄
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ABTS™ Chromophore SDS

      Title

      Safety Data Sheet (SDS) 

      ABTS™ Chromophore Certificates of Analysis

      TitleLot Number
      194434

      References

      Reference overview
      Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry 39, 13201.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-September-2020 JSW
      Synonyms2,2ʹ-Azino-bis(3-ethylbenzthiazoline-6-sulfonic Acid)
      DescriptionSingle component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.
      FormClear to very pale yellow solution
      FormulationContains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
      Recommended reaction conditionsSuggested procedure for use of ABTS in HRP-based ELISAs: 1. Complete all required incubations with antibodies and HRP-labeled probes. 2. Wash plate wells at least 4 times with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween®-20 detergent. 3. After the final wash, shake and blot all residual buffer from the wells. 4. Add 100 µl of ABTS Solution to each well and incubate at room temperature for up to 30 min. (If a kinetic study is desired, readings at 405 nm may be taken at intermediate intervals.) Note: Optimal incubation times may vary depending on the amount of HRP present. If color develops too quickly, zero order kinetics will not prevail. Dilution of the probe, antibody, or HRP-labeled reagent may be required. 5. Add 100 µl of stop solution, mix well, and read absorbance at 405 nm. [A 0.625 M oxalic acid solution is recommended as a stopping solution, as the colored product is most stable with this reagent (at least 1 h). Other acids may be employed (0.625 M oxalic acid > 0.5 M H2SO4 > 0.5 M HCl). 1% SDS may also be used as a stopping solution, provided that it is pure and clear (impure or turbid SDS solutions may interfere with absorbance readings).] Note: Variations in time, reagent volumes, and temperature may require further standardization by the user.
      CAS number28752-68-3
      Chemical formulaC₁₈H₁₈N₄O₆S₄
      Preservative≤0.1% sodium azide
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
      Toxicity Irritant
      ReferencesRodgriquez-Lopez, J.N., et al. 2000. Biochemistry 39, 13201.