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05-1050 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

05-1050
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, ICC, ELISA, IHC, IF, FC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1050
      Brand Family Upstate
      Trade Name
      • Upstate
      Description4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
      Background InformationThe development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
      References
      Product Information
      FormatPurified
      Control
      • Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      PresentationProprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide.
      Liquid at 2-8°C.
      Quality LevelMQ100
      Applications
      Application4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      • Immunohistochemistry
      • Immunofluorescence
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesImmunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
      Biological Information
      Immunogen4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
      Clone4G10®
      HostMouse
      SpecificityTyrosine-phosphorylated proteins from all species
      IsotypeIgG2b
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

      Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b) SDS

      Title

      Safety Data Sheet (SDS) 

      4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b) Certificates of Analysis

      TitleLot Number
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2116028 2116028
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2023921 2023921
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 1958115 1958115
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2006534 2006534
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2189938 2189938
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1535707 DAM1535707
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM165529 DAM165529
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1713267 DAM1713267
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1739309 DAM1739309
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1823498 DAM1823498

      References

      Reference overviewApplicationSpeciesPub Med ID
      Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex.
      Poulter, NS; Pollitt, AY; Davies, A; Malinova, D; Nash, GB; Hannon, MJ; Pikramenou, Z; Rappoport, JZ; Hartwig, JH; Owen, DM; Thrasher, AJ; Watson, SP; Thomas, SG
      Nature communications  6  7254  2015

      Show Abstract
      26028144 26028144
      Digital microfluidic assay for protein detection.
      Mok, J; Mindrinos, MN; Davis, RW; Javanmard, M
      Proceedings of the National Academy of Sciences of the United States of America  111  2110-5  2014

      Show Abstract
      24449893 24449893
      Angiotensin II induces membrane trafficking of natively expressed transient receptor potential vanilloid type 4 channels in hypothalamic 4B cells.
      Saxena, A; Bachelor, M; Park, YH; Carreno, FR; Nedungadi, TP; Cunningham, JT
      American journal of physiology. Regulatory, integrative and comparative physiology  307  R945-55  2014

      Show Abstract
      25080500 25080500
      SILAC-based quantitative proteomic analysis of human lung cell response to copper oxide nanoparticles.
      Edelmann, MJ; Shack, LA; Naske, CD; Walters, KB; Nanduri, B
      PloS one  9  e114390  2014

      Show Abstract
      Western Blotting25470785 25470785
      Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells.
      Bollu, LR; Ren, J; Blessing, AM; Katreddy, RR; Gao, G; Xu, L; Wang, J; Su, F; Weihua, Z
      Cell cycle (Georgetown, Tex.)  13  2415-30  2014

      Show Abstract
      25483192 25483192
      Themis sets the signal threshold for positive and negative selection in T-cell development.
      Fu, G; Casas, J; Rigaud, S; Rybakin, V; Lambolez, F; Brzostek, J; Hoerter, JA; Paster, W; Acuto, O; Cheroutre, H; Sauer, K; Gascoigne, NR
      Nature  504  441-5  2013

      Show Abstract
      Immunoprecipitation24226767 24226767
      Localization and identification of sumoylated proteins in human sperm: excessive sumoylation is a marker of defective spermatozoa.
      Vigodner, M; Shrivastava, V; Gutstein, LE; Schneider, J; Nieves, E; Goldstein, M; Feliciano, M; Callaway, M
      Human reproduction (Oxford, England)  28  210-23  2013

      Show Abstract
      Western Blotting23077236 23077236
      Antibodies against low-density lipoprotein receptor-related protein 4 induce myasthenia gravis.
      Shen, C; Lu, Y; Zhang, B; Figueiredo, D; Bean, J; Jung, J; Wu, H; Barik, A; Yin, DM; Xiong, WC; Mei, L
      The Journal of clinical investigation  123  5190-202  2013

      Show Abstract
      24200689 24200689
      Mer or Axl receptor tyrosine kinase inhibition promotes apoptosis, blocks growth and enhances chemosensitivity of human non-small cell lung cancer.
      Linger, RM; Cohen, RA; Cummings, CT; Sather, S; Migdall-Wilson, J; Middleton, DH; Lu, X; Barón, AE; Franklin, WA; Merrick, DT; Jedlicka, P; DeRyckere, D; Heasley, LE; Graham, DK
      Oncogene  32  3420-31  2013

      Show Abstract
      22890323 22890323
      Wnt proteins regulate acetylcholine receptor clustering in muscle cells.
      Zhang, B; Liang, C; Bates, R; Yin, Y; Xiong, WC; Mei, L
      Molecular brain  5  7  2012

      Show Abstract
      22309736 22309736

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      Life Science Research > Antibodies and Assays > Primary Antibodies