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480708 α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

480708
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480708-500MIU
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      Plastic ampoule 500 miu
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      Description
      OverviewRecombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.
      Note: 1 mU = 1 milliunit.
      Catalogue Number480708
      Brand Family Calbiochem®
      SynonymsAcetylneuraminyl Hydrolase, Sialidase
      References
      ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.
      Product Information
      CAS number9001-67-6
      Activity≥10 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
      EC number3.2.1.18
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥250 units/mg protein
      Physicochemical Information
      ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSQQ3450000
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      480708

      References

      Reference overview
      Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-August-2008 RFH
      SynonymsAcetylneuraminyl Hydrolase, Sialidase
      DescriptionRecombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions50 mM sodium phosphate buffer, pH 6.0.
      CAS number9001-67-6
      RTECSQQ3450000
      EC number3.2.1.18
      ContaminantsN-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
      Specific activity≥250 units/mg protein
      Activity≥10 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Harmful
      ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.