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407321 γ-Interferon, Rat, Recombinant, E. coli

407321
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407321-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
      Catalogue Number407321
      Brand Family Calbiochem®
      References
      ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
      Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
      Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.5X PBS, pH 7.2.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityReported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
      Purity≥95% by RP-HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      γ-Interferon, Rat, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      γ-Interferon, Rat, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      407321

      References

      Reference overview
      Alvarado, J., et al. 2005. Immunol. Lett. 99, 109.
      Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
      Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-July-2010 JSW
      DescriptionRecombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
      FormLyophilized
      FormulationLyophilized from 0.5X PBS, pH 7.2.
      Purity≥95% by RP-HPLC
      Biological activityReported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
      SolubilityReconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
      Storage -20°C
      Protect from moisture
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
      Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
      Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.