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MILLIPLEX® Multiplex Assays Using Luminex®

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As your research uncovers the significant role of more and more biomarkers in both normal and disease states, you need a reliable partner for quality assays and consistent performance. Our MILLIPLEX® portfolio of immunoassays is the largest portfolio of multiplex biomarker assays, based on Luminex® xMAP® technology, offering you consistent, high-quality results, so that you can do your best work while saving time, labor and cost.

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Already know your analytes of choice? Start designing and purchasing your own kits now!

Choose Customizable Panels & Premixed Kits – OR – Cell Signaling MAPmates™ Assays.    Start Now

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MILLIPLEX® Analyte Kit Finder

Use the kit finder below to choose the correct assays for your biomarkers of interest, so you can obtain the best quality data the first time.
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MILLIPLEX® Multiplex Assays for Research Applications in COVID-19

MILLIPLEX® multiplex immunoassays allow researchers to better understand the immune response to SARS-CoV-2 by simultaneously quantitating numerous cytokines and additional critical analytes. Discover more about our MILLIPLEX® COVID-19 applications, view our on-demand webinars demonstrating how MILLIPLEX® multiplex assays have been used in COVID-19 research, and read a growing number of published COVID-19 studies using MILLIPLEX® kits.
For Research Use Only. Not For Use In Diagnostic Procedures.
•	MILLIPLEX® COVID-19 Applications

48-Plex Human Cytokine Panel A

Larger and customizable! The MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A combines tests for 48 individual immune factors that previously have not been together in a single panel, while also allowing you choose any combination of analytes, or select a 48-plex or a 38-plex premixed kit. Standard Curves have broad and fixed ranges for lot-to-lot consistency, optimized for each analyte. This is the largest kit in the MILLIPLEX® portfolio of protein immunoassays.
New Human Cytokine Panel

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Analyte Quarterly Find the perfect set of assays!

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Download the MILLIPLEX® Tips & Tricks Brochure
Tips and TricksThe Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX®

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Need Tips on Optimizing your Multiplex Assays?
Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/Sonali-Nayak-headshot.jpgRead the SelectScience Interview with Sonali Nayak, Bioscience Technical Services Manager, MilliporeSigma:
Discover How Multiplex Assays are Advancing Biomedical Research: Top tips to optimize your analyses

Get Sonali Nayak’s take on how to best run your MILLIPLEX® assays.

Read Article

MILLIPLEX® Kit Verification and Quality Assurance Criteria

We carefully develop and verify every MILLIPLEX® kit and evaluate performance criteria including cross-reactivity, sensitivity, specificity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). We write the protocol with assay performance and your workflow in mind, making it easy to find the information you need.

Each MILLIPLEX® kit includes all necessary reagents to prepare the assay to read on your Luminex® instrument, just add your sample and water:
  • Selectable capture antibody-conjugated magnetic beads in solution
  • A premixed standard cocktail allowing you to easily rehydrate and prepare your serial dilutions
  • Two premixed quality control cocktails (QCs) to qualify assay performance
  • A premixed detection antibody cocktail designed to yield consistent analyte profiles within panel
  • A prediluted optimized concentration of Streptavidin Phycoerythrin (SAPE)
  • An optimized serum matrix is included for kits verified for serum or plasma samples, to mimic native sample environment
  • Bead diluent containing proprietary blocking reagents to reduce non-specific binding to the antibodies in the assay
  • 10X wash buffer and assay buffer optimized specifically for the kit
  • 96- or 384-well plate (depending on assay format) with two plate sealers

MILLIPLEX® cell signaling kits have a different format to support the use of cell lysate samples.

If you require analytes that are not in our portfolio, or different combinations of analytes, please visit our Custom Immunoassay Development Services page.

Related Resources: Brochures | Application Notes | Publications

How to Decide Which MILLIPLEX® Assay Is Best for Your Research

In Method Comparison tests (comparing ELISA and MILLIPLEX® assays), while the absolute values are not exactly the same, the results do correlate. Hence when switching from one assay method to another, a correction factor may be used when comparing with past data. In most situations the dynamic range and the sensitivity of the assay may be better with the MILLIPLEX® kits.

Questions? Visit our MILLPLEX® Frequently Asked Questions page. If you have a specific question, or one we did not answer, please contact our Technical Support Team directly.

Request the Analyte Quarterly brochure for a complete, updated list of assays.

Multiplex Customer Testimonial

What are researchers saying about MILLIPLEX® Quality?

“I have used MilliporeSigma MILLIPLEX® kits for the past four years. Initially I started using these kits to multiplex a specific set of markers, but given their reliability, consistency, and ease of use, I have transitioned all my experiments away from traditional ELISAs. Beyond just being a great product, MilliporeSigma's customer and technical support is at the top of the industry. The combination of superior product with award-winning support has extended the capabilities of my research, allowing me to answer new novel research questions. I consider MILLIPLEX® kits an important component of my research toolbox.” 

Brian McFarlin, PhD, FACSM, University of North Texas, Denton, Texas

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