Transfection & Protein Expression Overview
Successful delivery of nucleic acids and protein in eukaryotic cells requires a transfection protocol that maximizes transfection efficiency, minimizes cytotoxicity, and results in the desired level of gene expression or silencing. MilliporeSigma recognizes that there is not a one-size-fits-all solution to your transfection needs. We provide the breadth of transfection reagents necessary to give you the freedom to design the perfect experiment.
Our reagents provide superior alternatives to a wide variety of other DNA transfer techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Simplified protocols, including no media changes for most reagents and cell types, have been optimized, allowing more time for research.
With MilliporeSigma’s protein expression platforms, you can choose the proteins you purify to answer the precise biological questions you’re tackling. Don’t let limitations like solubility, yield, or toxicity influence your choice of proteins to study. With the expertise of Novagen®, the household name in cloning and recombinant protein expression technology, MilliporeSigma makes it possible to elucidate meaningful functions of even the most intractable gene products.