|Featured Technique: Proliferation Assays
|Evaluation of cell proliferation is essential for studies of most biological processes and for many cellbased assays. The traditional method for detection of cell proliferation has been the measurement of [3H] thymidine incorporation as cells enter S phase, and subsequent quantification of [3H] thymidine, as performed by scintillation counting. This technology is slow, labor-intensive and has several limitations, including the handling and disposal of radioisotopes and the necessity of expensive equipment. Merck Millipore has developed multiple technologies for biomolecular detection and cellular analysis that offer significant advantages over [3H] thymidine incorporation for quantifying cell proliferation with speed, precision, and accuracy. These include the use of non–radioactive reagents such as EdU, BrdU, WST-1, and MTT.
|Featured Solution: NEW EdU Cell Proliferation Assays
|The use of EdU (5-ethynyl-2’-deoxyuridine) as a thymidine nucleoside analog is a significant improvement compared to the classical BrdU and 3[H] thymidine cell proliferation assays. In contrast to BrdU assay kits, the EdU cell proliferation assays are not antibody based and do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Cell Proliferation assays measure the incorporation of EdU into newly synthesized DNA with click chemistry for detection by fluorescence microscopy and flow cytometry applications.
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|Related Proliferation Assays
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