DNA Damage & TUNEL Assays
Product Highlight:ApopTag Fluorescein In Situ Apoptosis Detection Kit
|The ApopTag Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining and results can be analyzed using either flow cytometry or fluorescence microscopy.
The ApopTag Fluorescein In SituApoptosis Detection Kit has been tested for specific staining in these model systems: (a) human normal peripheral blood lymphocytes induced with dexamethasone as stained in cytospins, (b) rat regressing mammary gland as stained in formalin-fixed, paraffin-embedded sections, and (c) human leukemic peripheral blood lymphocytes induced with camptothecin, as stained in cell suspensions and used for quantitative flow cytometry.
Product Highlight:ApopTag ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & II)
|While conventional in situ detection techniques such as ISEL (Klenow DNA polymerase), TUNEL (terminal deoxynucleotidyl transferase, TdT) and ISNT (DNA Polymerase I) are useful in detecting internucleosomal DNA cleavage, they do not differentiate DNase Type I and DNase Type II cleavage which results from the activation of apoptotic endonucleases. ApopTag ISOL Kits facilitate the differentiation of apoptotic cells from necrotic or transiently damaged cells.
The ApopTag ISOL Dual Fluorescence Kit utilizes a proprietary double hairpin, dual fluorescently labeled oligonucleotide labeling process to detect and distinguish between typical apoptotic DNA breaks induced by either DNase I or DNase II enzyme activities. The Vaccinia Topoisomerase I mediated ligation reaction is adaptable for staining paraffin-embedded tissue, frozen tissue sections, cell suspensions, and adherent cells. In situ staining for DNA fragmentation (as in the ISOL method) is both a means of detection for rare cells and an analytical test of those cells' DNA,
Product Highlight:Apo-Direct TUNEL Assay Kit
|The Apo-Direct Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry. The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
|ApopTag Red In Situ Apoptosis Detection Kit
|ApopTag Plus Peroxidase In Situ Apoptosis Kit
|ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit
|ApopTag Peroxidase In Situ Apoptosis Detection Kit
|ApopTag Fluorescein In Situ Apoptosis Detection Kit
|ApopTag Plus In Situ Apoptosis Fluorescein Detection Kit
|Apo-Direct TUNEL Assay Kit
|ApopTag ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & II)
|FragEL DNA Fragmentation Detection Kit, Colorimetric - Klenow Enzyme
|FragEL DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme
|FragEL DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme