Neurite Outgrowth Assays
|Research Paper Spotlight|
|Spatially Restricted Exposure of Toxins to Neurons and its Impact on Axonal Degeneration Using AXIS™ System.
Investigations into neurotoxic effects have been hampered by the lack of an in vitro test system capable of separating axons from their cell bodies. Using a novel microfluidic AXonal Isolation System (AXIS System) we were able to spatially isolate axons so they could be directly and specifically exposed to neurotoxins.
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Neurite Outgrowth Quantification AssayMilliporeSigma’s NS230 Neurite Outgrowth Assay Plus Kit (3 µm) utilizes microporous tissue culture insert technology from MilliporeSigma and is based on the use of Millicell inserts which contain a permeable membrane with 3-µm pores. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm.
AXIS™ Axon Investigation System:An advanced platform for spatially controlled neurite outgrowth and analysis
AXIS Axon Isolation Device is a two chamber system, each composed of two wells and an interconnected channel, separated by a set of microgrooves. The hydrostatic pressure formed by volume differential between chambers induces fluidic isolation of the solution on the low volume side of the device.