2-D separation of single protein digests on ProteoChrom® HPTLC cellulose sheets
A. ProteoChrom® HPTLC cellulose sheet followed by fluorescamine staining

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-TNET-Application_examples_blue.jpg 
 
B. ProteoChrom® HPTLC cellulose sheet followed by ninhydrin staining

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-TNET-Application_examples_red.jpg 
Sample volume 5 µL
Concentration 2 mg/mL
Application system Automatic TLC sampler 4 (CAMAG)
Mobile phase 1st dimension:
2-butanol/pyridine/acetic acid/ water (30/20/6/24)
2nd dimension:
2-butanol/pyridine/ammonia (25%)/water (39/34/10/26)
Migration distance 5 cm
Migration time 1st dimension: 44 min
2nd dimension: 50 min
Staining A: Fluorescamine B: Ninhydrin