16 PAH acc. to EPA 610/550 + Benzo(e)pyrene + Perylene by Fluorescence Detection

Column	LiChrospher® PAH (5 μm) LiChroCART® 250-4
Mobile phase	A: Acetonitrile B: Water
Gradient	0 - 3 min 60% A 3 -15 min 60% A - 100% A 15 - 50 min 100% A
Flow rate	1.0 mL/min
Detection	Programmed fluorescence
Temperature	20°C
Injection volume	10 μl
Peak No.	Ex nm	Em mm	Sample	A	B
1, 3, 4	280	330	1. Naphthalene	2.00 μg/mL	100.0 ng/mL
5	246	370	2. Acenaphthylene	1.54 μg/mL	n.n.
6	250	406	3. Acenaphthene	2.06 μg/mL	103.0 ng/mL
7	280	450	4. Fluorene	0.48 μg/mL	24.0 ng/mL
8	270	390	5. Phenanthrene	0.35 μg/mL	17.5 ng/mL
9, 10	265	380	6. Anthracene	0.08 μg/mL	4.0 ng/mL
11 - 15	290	430	7. Fluoranthene	0.77 μg/mL	4.0 ng/mL
16,17	290	410	8. Pyrene	0.85 μg/mL	42.5 ng/mL
18	300	500	9. Benzo(a) anthracene	0.41 μg/mL	20.5 ng/mL
			10. Chrysene	0.37 μg/mL	18.5 ng/mL
			11. Benzo(e)pyrene	1.00 μg/mL	37.0 ng/mL
			12. Benzo(b) fluoranthene	0.42 μg/mL	21.0 ng/mL
			13. Perylene	1.00 μg/mL	36.0 ng/mL
			14. Benzo(k) fluoranthene	0.42 μg/mL	23.5 ng/mL
			15. Benzo(a)pyrene	0.49 μg/mL	24.5 ng/mL
			16. Dibenzo(a,h) anthracene	0.36 μg/mL	18.0 ng/mL
			17. Benzo(g,h,i) perylene	0.37 μg/mL	18.5 ng/mL
			18. Indeno(1,2,3-c,d)pyrene	0.43 μg/mL	21.5 ng/mL