Angiotensin I and II


Column LiChrospher® WP 300 RP-18 (5 μm) LiChroCART® 250-4 Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-CIA LiChrospher WP RP18 Angiotensin-480x491-02242014.png
Mobile phase A: Water + 0.1% TFA
B: Acetonitrile + 0.1%TFA
Gradient 20 min 20% B - 60% B
Flow rate 0.6 mL/min
Detection UV 214
Temperature Room temperature
Injection volume 100 μl
Sample 1. Angiotensin II (human)
2. Angiotensin I (human) each 1 mg/mL