Environmental Analysis: Phenols

Column	LiChrospher® 60 RP-select B (5 μm ) LiChroCART® 250-4
Mobile phase	A:Water LiChrosolv® + 1% Acetic acid (96%) B: Acetonitrile LiChrosolv® + 1% Acetic acid (96%)
Gradient	0 - 10 min 30% B 10 - 28 min 30 - 80% B 28 - 29 min 80 - 30% B 29 - 35 min 30% B
Flow rate	1.0 mL/min
Detection	Diode array 0.0 min 6.0 min 8.5 min 11.0 min 16.0 min 19.5 min 22.0 min 25.0 min	Detector 362 nm 273 nm 319 nm 278 nm 283 nm 269nm 293 nm 304 nm
Temperature	30 °C
Injection volume	100 μl
Sample	1. Picric acid 2. Phenol 3. 4-Nitrophenol 4. 2-Chlorophenol 5. 2,4-Dinitrophenol 6. 2-Nitrophenol 7. 2,4-Dimethylphenol 8. 4-Chloro-3-methylphenol 9. 2-Methyl-4,6-dinitrophenol 10. 2,4,6-Trichlorophenol 11. Pentachlorophenol