Nucleotides


Column LiChrospher® 100 RP-8 (5 μm) Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-CIA LiChrospher RP8 nucleotides-480x672-02212014.png
Mobile phase Acetonitrile/0.05 M phosphate buffer pH 6.5 90/10 (v/v) + 0.001 mol/l TBAHSO4
Flow rate 1.5 mL/min
Detection UV 254 nm
Sample 1. NAD
2. Adenosine
3. NADH
4. NADPH