Dmb-dipeptides are unique derivatives for enhancing the synthesis of hydrophobic, aggregated sequences that contain glycine. They work in exactly the same way as pseudoproline dipeptides by exploiting the natural propensity of N-alkyl amino acids to disrupt the formation of the secondary structures during peptide assembly. Their use results in better and more predictable acylation and deprotection kinetics, enhanced reaction rates, and improved yields, purities and solubilities of crude products.
Dmb dipeptides are extremely easy to use: simply substitute a Gly residue together with the preceding Ala, Ile, Asp, Gly, Leu or Val residue in the peptide sequence with the appropriate Dmb dipeptide. They are fully compatible with standard coupling methods such as PyBOP® or HBTU, since unlike analogous Hmb-based derivatives, they cannot form cyclic lactones.