Cellulose Plates

Precise Analysis of Polar Substances

The organic sorbent cellulose is particularly suitable for the separation of hydrophilic substances by partition chromatography. MilliporeSigma offers cellulose plates for demanding high-performance separations. Based on microcrystalline cellulose, the TLC plates are ideal for standard separations. Whereas the HPTLC plates use high-purity, rod-shaped microcrystalline cellulose, resulting in a highly reduced diffusion of analytes for demanding, high-performance separations.

We also provide special PEI cellulose plates (polyethylenimine-modified cellulose), which act as strong basic anion exchangers. This makes them the best choice for analyzing substances with exchange-active ionic groups, such as amino acids, peptides, nucleotides and nucleosides.

Cellulose plates are available with or without fluorescent indicators. They utilize a special fluorescent pigment that is stimulated to intense blue fluorescent emission by long-wave UV light of 366 nm and by short-wave UV light at 254 nm.

Applications

Typical applications of cellulose plates include the analysis of amino acids, carbohydrates, phosphates, nucleic acids and nucleic acid derivatives for:

• Detection of significantly high increase of amino acids in clinical laboratories
• 2-dimensional separations such as amino acid “fingerprints”
• Metabolic studies