Classical Silica HPTLC Plates

Higher Speed and Sensitivity

MilliporeSigma’s classical silica HPTLC plates enable fast, quantitative analyses of complex samples for manual or automated use. These plates offer higher speed and sensitivity than conventional TLC plates – making them the best choice for sophisticated separations.

Features and Benefits

• Faster analysis, only 3 – 20 min for optimum separations
• 5 – 10 fold higher sensitivity than classical TLC
• Highly reproducible, focused bands for quantitative analysis
• Gold standard for instrumental automation

Smaller Particles for Advanced Separations

Our HPTLC plates use an optimized silica gel 60 sorbent with a particle size of only 5 - 6 µm, compared to the 10 - 12 µm used in classical TLC. This enables a higher packing density and smoother surface. What’s more, band diffusion is reduced, yielding especially compact sample zones or spots. The smaller particle size and thinner layer (200 µm or 100 µm) result in significantly higher sensitivity and faster analysis.

Application Areas

HPTLC plates are ideal for highly demanding quantitative analyses, such as:

• Identity testing in the analysis of herbal medicines
• Highly sophisticated quantitative separations of pharmaceutical drugs using instrumental analysis
• Quality control or purity testing of complex samples in pharmaceutical drugs
• Trace analysis of contaminants in food

Comparison of the particle size distribution of TLC and HPTLC plates

Complete Range

MilliporeSigma’s classical silica HPTLC plates are available with glass or aluminum backing in a variety of formats to suit your requirements. Fluorescent indicators are also available: green fluorescent F₂₅₄, or blue fluorescent acid-stable F₂₅₄s. Both indicators fluoresce in UV light at an excitation wavelength of 254 nm.

AMD HPTLC plates

With an extra-thin layer of only 100 μm, AMD HPTLC plates are designed for even more demanding, automated multiple development (AMD) applications. The technique combines repeated development of the plate in the same direction with reproducible gradient elution. AMD HPTLC plates provide extremely narrow bands, allowing the complete resolution of up to 40 components over a distance of only 60 mm. The special properties of AMD HPTLC plates are highly beneficial for complex investigations, like the qualitative and quantitative analysis of pesticides.

Premium Purity HPTLC Plates

Premium purity HPTLC plates are optimized for high-performance, completely contamination-free separations, such as demanding pharmacopoeia applications. The plates are wrapped in plastic-coated aluminum foil, which prevents deposition of plasticizers that could appear as unknown extra zones when using medium-polar solvent systems.