Cellulose HPTLC Plates

High-Performance Separations of Polar Compounds

Cellulose is an organic sorbent, which is particularly suitable for the separation of hydrophilic substances by partition chromatography. MilliporeSigma’s high-quality cellulose plates include classical TLC or HPTLC grades, and facilitate a wide range of separations. Both plates are available with or without a special fluorescent indicator, which is stimulated to intense blue fluorescent emission at long-wave UV light of 366 nm, and at short-wave UV light of 254 nm.

TLC cellulose layers: Based on microcrystalline cellulose for standard separations

HPTLC cellulose layers: Based on high-purity, rod-shaped microcrystalline cellulose, resulting in a highly reduced diffusion of analytes for critical high-performance separations

Features and Benefits

• Ideal for partition chromatography of hydrophilic substances
• High-performance separations due to greatly reduced diffusion of analytes
• Easier detection through intense fluorescent indicator

Application Areas

Typical applications of cellulose plates include the analysis of amino acids, carbohydrates, phosphates, nucleic acids and nucleic acid derivatives for:

• Two-dimensional separations, such as amino acid “fingerprints”
• Metabolic studies

Separation of Phosphates

Compounds	1. (NaPO3)3 2. Na5P3O10 3. Na4P2O7 4. Na2HPO4 all 0.2%
Sample volume	250 nL
Mobile phase	16% trichloroacetic acid diocane sol. ammonia in 1 L water; 70/30
Migration distance	7 cm
Detection	586 nm, TLC/HPTLC scanner, Camag