L1 US Pharmacopoeia Columns |
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MilliporeSigma offers a broad range of HPLC columns that are fully compliant with USP (United States Pharmacopoeia) specifications. L1 USP columns are described as octadecyl silane, chemically linked to porous silica or ceramic micro particles. Our high-quality L1 USP products deliver reliable, reproducible results – time after time. A perfect example is: Chromolith® RP-18 endcapped.
Produced from a continuous piece of porous silica using a sol-gel process, Chromolith® monolithic HPLC columns possess a defined bimodal pore structure with macro and mesopores in the micro and nanometer range. The high permeability and porosity of the silica skeleton, and the resulting low back pressure allow for more flexible flow rates than particle-packed columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.
Chromolith® RP-18 endcapped HPLC columns combine high throughput and lower matrix sensitivity with an extremely long column lifetime. Thanks to their high-purity silica base, they minimize the negative effects of trace metals. Furthermore, the columns are chemically modified with n-alkyl chains, which possess a high ligand density, and fully endcapped in order to reduce the effects of unmodified silanol groups.
The chemical basis of Chromolith® RP-18 endcapped columns – from starting materials to surface modifications – is identical to high-end, conventional packed columns. Hence, their selectivity is comparable to high-quality C18 endcapped reversed-phase particle packed columns. This allows the use of standard methods when developing new protocols.
Method transfer from particulate to monolithic Chromolith® columns is simple and seamless. This is because the chromatographic selectivity of Chromolith® columns is very similar to that of many modern particulate HPLC columns (e.g. Purospher®). Chromolith® columns are now included in the US Pharmacopoeia in categories L1, L3 and L7.
Chromolith® Flash RP-18 endcapped is the fastest HPLC column on the market. Due to the very short length of the column, its number of theoretical plates is sufficient for easy separations.
These short, fast columns are not only perfect for rapid screening of samples during in-process controls, but also for laboratories specialized in research or organic synthesis (e.g. combinatorial chemistry).
This column is perfect for use as a routine analytical tool in quality control laboratories, or in research laboratories where more complex mixtures are being analyzed.
Chromolith® Performance RP-18e
The validation kit includes three columns from three different production batches, allowing you to easily compare batch-to-batch reproducibility and quality. This makes the validation kit an ideal tool for quality control and validation laboratories.
Standard Chromolith® columns have 2 μm macropores, and an efficiency equal to 4.5 μm particulate columns. These columns allow very high flow rates, extreme throughputs, and the analysis of relatively dirty samples. In contrast, Chromolith® HighResolution (HR) columns possess 1.15 μm macropores. This results in higher efficiency and improved peak shape. Although this causes higher back pressure, it is still less than half that of any particulate column of the same dimensions.
MilliporeSigma offers an extensive range of services, information and tools to support you in your analysis. Use the links below to learn more.
