565834 sFRP-3, His•Tag®, Mouse, Recombinant

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
565834
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      565834-10UG
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      Limited Quantities Available
      Available
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          Contact Customer Service

          		 Glass bottle 10 μg
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          Description
          OverviewRecombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
          Catalogue Number565834
          Brand Family Calbiochem®
          References
          ReferencesHoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Applications
          Biological Information
          Biological activityProliferation Assay:
          ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.

          Functional ELISA:
          50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotxin: ≤1 EU/µg
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          sFRP-3, His•Tag®, Mouse, Recombinant SDS

          Title

          Safety Data Sheet (SDS) 

          sFRP-3, His•Tag®, Mouse, Recombinant Certificates of Analysis

          TitleLot Number
          565834

          References

          Reference overview
          Hoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision21-Sepmtember-2011 RFH
          DescriptionRecombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotxin: ≤1 EU/µg
          Biological activityProliferation Assay:
          ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.

          Functional ELISA:
          50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
          SolubilityReconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesHoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.