71228 pSTBlue-1 AccepTor™ Vector Giga Kit - Novagen

71228
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          Description
          Overview

          The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.

          The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.

          Catalogue Number71228
          Brand Family Novagen®
          Features and benefits• No restriction enzymes or special primers
          • Direct ligation of PCR product with vector
          • Compatible with polymerases that leave single 3´-dA overhangs
          • Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
          • Simple protocol takes as little as 40 minutes from PCR product to plating transformants
          References
          Product Information
          Components
          DeclarationThis product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.
          Quality LevelMQ100
          Applications
          Biological Information
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          Toxicological Information
          Safety Information according to GHS
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          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
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          Supplemental Information
          Specifications

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          Categories

          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Kits