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71339 pIEx™-7 Ek/LIC Vector Kit - Novagen

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Catalog NumberPackaging Qty/Pack
71339-3 Plastic ampoule 20 rxn
OverviewThe pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1).
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number71339
Brand Family Novagen®
SynonymsInsectDirect™ pIEx™-7 Ek/LIC Vector Kit
ReferencesKim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
Product Information
1 µgEk/LIC Vector
25 unitsT4 DNA Polymerase, LIC-qualified
50 µl25 mM EDTA
40 µl25 mM dATP
8 µlEk/LIC β-gal Control Insert
100 µl100 mM DTT
50 µl10X T4 DNA Polymerase Buffer
1.5 mlNuclease-free Water
5 × 2 mlSOC Medium
10 µlTest Plasmid
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
71339-3 04055977253269


pIEx™-7 Ek/LIC Vector Kit - Novagen Certificates of Analysis

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Reference overview
Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.


Radiance! Multisystem Protein Expression


  • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
  • User Protocols

    TB163 Ek/LIC Cloning Kits

    Vector Map

    TB399VM pIEx-7 Ek/LIC Vector Map

    Vector Sequence

    pIEx-7 Ek/LIC Vector Sequence