Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on More

70755 pET Expression System 15b - Novagen

Purchase on Sigma-Aldrich


Replacement Information


Catalog NumberPackaging Qty/Pack
70755-3 Glass bottle 1 ea
OverviewpET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 15b
The pET-15b vector carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB045VM.

Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number70755
Brand Family Novagen®
Product Information
Fusion tagHis•Tag
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
70755-3 04055977273175


pET Expression System 15b - Novagen Certificates of Analysis

TitleLot Number


  • Nicola G. Jones, et al. (2008) Structure of a glycosylphosphatidylinositol-anchored domain from a Trypanosome variant surface glycoprotein. Journal of Biological Chemistry 283, 3584-3593.
  • Lori Buetow, et al. (2007) Structure and reactivity of LpxD, the N-acyltransferase of lipid A biosynthesis. Procedings of the National Academy of Science 104, 4321-4326.
  • Gintaras Deikus and David H. Bechhofer. (2007) Initiation of decay of Bacillus subtilis trp leader RNA. Journal of Biological Chemistry 282, 20238-20244.
  • Lilia Kaustov, et al. (2007) The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain of P53. Journal of Biological Chemistry 282, 11300-11307.
  • Julia M. Malleier, et al. (2007) Regulation of protein C inhibitor (PCI) activity by specific oxidized and negatively charged phospholipids. Blood 109, 4769-4776.
  • Yoshihiro Nakamura, et al. (2007) Crystal structure of the human BRD2 bromodomain: insights into dimerization and recognition of acetylated histone H4. journal of Biological Chemistry 282, 4193-4201.
  • Mette Praetorius-Ibba, et al. (2007) Functional association between three archaeal aminoacyl-tRNA synthetases. Journal of Biological Chemistry 282, 3680-3687.
  • Hideaki Unno, et al. (2007) Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis. Journal of Biological Chemistry 282, 15812-15822.
  • Andriy S. Yatsenko, et al. (2007) A putative Src homology 3 domain binding motif but not the C-terminal dystrophin WW domain binding motif is required for dystroglycan function in cellular polarity in Drosophila. Journal of Biological Chemistry 282, 15159-15169.
  • Zubair M. Ahmed, et al. (2006) The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15. Journal of Neuroscience 26, 7022-7034.
  • Fanny Boissier, et al. (2006) Further insight into S-adenosylmethionine-dependent methyltransferases: structural characterization of Hma, an enzyme essential for the biosynthesis of oxygenated mycolic acids in Mycobacterium tuberculosis. Journal of Biological Chemistry 281, 4434-4445.
  • Annika Grabbe and Jurgen Wienands. (2006) Human SLP-65 isoforms contribute differently to activation and apoptosis of B lymphocytes. Blood 108, 3761-3768.
  • Jeff W. Hill and Michele K. Evans. (2006) Dimerization and opposite base-dependent catalytic impairment of polymorphic S326C OGG1 glycosylase. Nucleic Acids Research 34, 1620-1632.
  • Lee M. Hoffart, et al. (2006) Direct spectroscopic detection of a C-H-cleaving high-spin Fe(IV) complex in a prolyl-4-hydroxylase. Procedings of the National Academy of Science 103, 14738-14743.
  • Dmitri Nashchekin, et al. (2006) A novel ded1-like RNA helicase interacts with the Y-box protein ctYB-1 in nuclear mRNP particles and in polysomes. Journal of Biological Chemistry 281, 14263-14272.
  • Adam Robertson, Steven R. Pattishall and Steven W. Matson. (2006) The DNA binding activity of mutL Is required for methyl-directed mismatch repair in Escherichia coli. Journal of Biological Chemistry 281, 8399-8408.
  • Yi-Hsien Su and Victor D. Vacquier. (2006) Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa. Molecular Biology of the Cell 17, 114-121.
  • Dewan Andrews, et al. (2005) Bacteriophage P22 tail accessory factor gp26 is a long triple-stranded coiled-coil. Journal of Biological Chemistry 280, 5929-5933.
  • Felice Arcuri, et al. (2005) The translationally controlled tumor protein is a novel calcium binding protein of the human placenta andregulates calcium handling in trophoblast cells. Biology of Reproduction 73, 745-751.
  • Janice Bramham, et al. (2005) Functional insights from the structure of the multifunctional C345C domain of C5 of complement. Journal of Biological Chemistry 280, 10636-10645.
  • Daniel M. Cohen, et al. (2005) Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. Journal of Biological Chemistry 280, 17109-17117.
  • Marcelo Comini, et al. (2005) Trypanothione synthesis in Crithidia revisited. Journal of Biological Chemistry 280, 6850-6860.
  • Aimee L. Eggler, et al. (2005) Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2. Proceedings of the National Academy of Sciences (USA) 102, 10070-10075.
  • Ralf Flaig, et al. (2005) Structural basis for the cell-specific activities of the NGFI-B and the Nurr1 ligand-binding domain. Journal of Biological Chemistry 280, 19250-19258.
  • Martino Forino, et al. (2005) Efficient synthetic inhibitors of anthrax lethal factor. Proceedings of the National Academy of Sciences (USA) 102, 9499-9504.
  • Peter Gin and Catherine F. Clarke. (2005) Genetic evidence for a multi-subunit complex in coenzyme Q biosynthesis in yeast and the role of the coq1 hexaprenyl diphosphate synthase. Journal of Biological Chemistry 280, 2676-2681.
  • Samia Hamimes, et al. (2005) RDM1, a novel RNA-recognition motif (RRM)-containing protein involved in the cell response to cisplatin in vertebrates. Journal of Biological Chemistry 280, 9225-9235.
  • Mitsumasa Hanaoka, et al. (2005) Glutamyl-tRNA mediates a switch in RNA polymerase use during chloroplast biogenesis. European Molecular Biology Organization Reports 6, 545-550.
  • Valerie Heurgue-Hamard, et al. (2005) The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 Is methylated by the product of the YDR140w gene. Journal of Biological Chemistry 280, 2439-2445.
  • Frank Hillger, et al. (2005) Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. Journal of Biological Chemistry 280, 14974-14980.
  • Ia-Wen Hsu, et al. (2005) Phosphorylation of Y14 modulates its interaction with proteins involved mRNA metabolism and influences its methylation. Journal of Biological Chemistry 280, 34507-34512.
  • Nadege Jamin, et al. (2005) Characterization of the cholesterol recognition amino acid consensus sequence of the peripheral-type benzodiazepine receptor. Molecular Endocrinology 19, 588-594.
  • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
  • Sung Hoon Kim, et al. (2005) A proteomic microarray approach for exploring ligand-initiated nuclear hormone receptor pharmacology, receptor-selectivity,and heterodimer functionality. Molecular and Cellular Proteomics 4, 267-277.
  • Siva Kumar Kolluri, et al. (2005) The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis. Procedings of the National Academy of Science 102, 2525-2530.
  • Beth T. Vander Kooi, et al. (2005) The glucose-6-phosphatase catalytic subunit gene promoter contains both positive and negative glucocorticoid response elements. Molecular Endocrinology 19, 3001-3022.
  • Zuzanna Kwade, et al. (2005) Identification of four adenosine kinase isoforms in tobacco by-2 cells and their putative role in the cell cycle regulated cytokinin metabolism. Journal of Biological Chemistry 280, 17512-17519.
  • Roberta Leonardi, et al. (2005) A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A. Journal of Biological Chemistry 280, 3314-3322.
  • Jun Li, et al. (2005) Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetra- and octa-peptide substrate libraries. Journal of Biological Chemistry 280, 28766-28774.
  • Ying Liu, Zhi-Xing Yao and Vassilios Papadopoulos. (2005) Cytochrome p450 17α hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells. Molecular Endocrinology 19, 1918-1931.
  • Ying-Jie Lu, St
  • User Protocols

    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    TB045VM pET-15b Vector Map