71327 pET-45b(+) DNA - Novagen

71327
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-45b plasmid features the ability to express fusion proteins with an N-terminal His•Tag® coding sequence that results in native protein after purification and cleavage. The plasmid contains a strong T7lac promoter, an amino-terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector-encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71327
      Brand Family Novagen®
      References
      References1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
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      Materials Information
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      Safety Information according to GHS
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      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
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      Documentation

      pET-45b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      71327

      References

      Reference overview
      1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

      Brochure

      Title
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

      Citations

      Title
    • Robert T. Glover, et al. (2007) The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacterium smegmatis. Journal of Bacteriology 189, 5495-5503.
    • Guifeng Sun, et al. (2007) Structural and functional analyses of the major outer membrane protein of Chlamydia trachomatis. Journal of Bacteriology 189, 6222-6235.
    • Jun Dai, et al. (2005) The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes and Development 19, 472-488.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB396VM pET-45b(+) Vector Map