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70939 pET-43.1a(+) DNA - Novagen

70939
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB288VM. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshA I or Sma I sites by cleaving the Nus•Tag fusion protein with Enterokinase or Thrombin, respectively.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70939
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
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      Documentation

      pET-43.1a(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      70939

      Brochure

      Title
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

      Citations

      Title
    • Kenneth Segers, et al. (2007) Design of protein-membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V. Procedings of the National Academy of Science 104, 12697-12702.
    • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
    • Ju-Ock Nam, et al. (2005) Regulation of tumor angiogenesis by fastatin, the fourth FAS1 domain of β-ig-h3, via α-v-β-3 integrin. Cancer Research 65, 4153-4161.
    • Aymeric Goyer, et al. (2004) Characterization and metabolic function of a peroxisomalsarcosine and pipecolate oxidase from Arabidopsis. Journal of Biological Chemistry 279, 16947-16953.
    • Lei Zheng, Ulrich Baumann and Jean-Louis Reymond. (2003) Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm. Journal of Biochemistry (Tokyo) 133, 577-581.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB288VM pET-43.1a-c(+) Vector Map

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      70940 pET-43.1b(+) DNA - Novagen Show Pricing & Availability

      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors