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71071 pET-41 Ek/LIC Vector Kit - Novagen

71071
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      Description
      OverviewDirectional PCR cloning into the most powerful E. coli expression vectors

      The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71071
      Brand Family Novagen®
      References
      Product Information
      Components
      Vector familypET
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-41 Ek/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number
      71071

      Brochure

      Title
      Radiance!" Multisystem Protein Expression

      Citations

      Title
    • Chun Dong, et al. (2006) Hemicentin assembly in the extracellular matrix is mediated by distinct structural modules. Journal of Biological Chemistry 281, 23606-23610.
    • Hoyee Leong, et al. (2005) Recruitment of histone deacetylase 4 to the N-terminal region of estrogen receptor alpha. Molecular Endocrinology 19, 2930-2942.
    • User Protocols

      Title
      TB055 pET System Manual
      TB163 Ek/LIC Cloning Kits
      TB184 Xa/LIC Cloning Kits

      Vector Map

      Title
      TB317VM pET-41 Ek/LIC Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors