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69952 pET-31b(+) DNA - Novagen

69952
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
69952-3 Plastic ampoule 10 μg
Description
OverviewThe pET-31b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein (1). The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB098).

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69952
Brand Family Novagen®
References
References1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
69952-3 04055977258813

Documentation

pET-31b(+) DNA - Novagen Certificates of Analysis

TitleLot Number
69952

References

Reference overview
1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.

Citations

Title
  • Graeme S. Garvey, Susan P. McCormick and Ivan Rayment. 2008. Journal of Biological Chemistry 283, 1660.
  • Kim, M-J., et al. 2007. Journal of Biological Chemistry 282, 14253.
  • Ames, J.B., et al. 2006. Journal of Biological Chemistry 281, 37237.
  • Beloqui, A., et al. 2006. Journal of Biological Chemistry 281, 22933.
  • Pimentel, C., et al. 2006. Protein Science 15, 628.
  • Baneres, J-L., et al. 2005. Journal of Biological Chemistry 280, 20253.
  • Thai, K., et al. 2005. Protein Science 14, 948.
  • Juan Carlos Rodriguez, Lilly Wong and Patricia A. Jennings. 2003. Protein Expression and Purification 28, 224.
  • User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual
    TB104 pET Peptide Expression System 31

    Vector Map

    Title
    TB098VM pET-31b(+) Vector Map

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    Categories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors