69743 pET-21d(+) DNA - Novagen

69743
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-21a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-24a-d(+) only by their selectable marker (ampicillin vs. kanamycin resistance). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB036). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69743
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Specifications

      Documentation

      pET-21d(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69743

      Citations

      Title
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    • Christian Zuber, et al. (2007) EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites. Procedings of the National Academy of Science 104, 4407-4412.
    • Petra Bjork, et al. (2006) Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites. Molecular Biology of the Cell 17, 32-42.
    • Josephine C. Ferreon, et al. (2005) Molecular determinants of TRIF proteolysis mediated by the hepatitis C virus NS3/4A protease. Journal of Biological Chemistry 280, 20483-20492.
    • George A. Kassavetis, et al. (2005) Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB. Proceedings of the National Academy of Sciences (USA) 102, 15406-15411.
    • Gaohua Liu, et al. (2005) High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases. Protein Science 14, 1597-1608.
    • Halina Offner, et al. (2005) Treatment of passive experimental autoimmune encephalomyelitis in SJL mice with a recombinant TCR ligand induces IL-13 and prevents axonal injury. Journal of Immunology 175, 4103-4111.
    • Daniel W. Schuerch, Elizabeth M. Wilson-Kubalek and Rodney K. Tweten. (2005) Molecular basis of listeriolysin O pH dependence. Proceedings of the National Academy of Sciences (USA) 102, 12537-12542.
    • Robert V. Stahelin, et al. (2005) Diacylglycerol-induced membrane targeting and activation of protein kinase C: mechanistic differences between PKCδ and Cε. Journal of Biological Chemistry 280, 19784-19793.
    • Atsushi Takabayashi, et al. (2005) Differential use of two cyclic electron flows around photosystem I for driving CO2-concentration mechanism in C4 photosynthesis. Proceedings of the National Academy of Sciences (USA) 102, 16898-16903.
    • Khang Thai, et al. (2005) Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins. Protein Science 14, 948-955.
    • Daniel A. Vallera, et al. (2005) A bispecific recombinant immunotoxin, DT2219, targeting human CD19 and CD22 receptors in a mouse xenograft model of B-cell leukemia/lymphoma. Clinical Cancer Research 11, 3879-3888.
    • B.-S. Youn, et al. (1997) Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3. Journal of Immunology 159, 5201-5202.
    • Masato Nakai, Kazuya Kinoshita and Toshiya Endo. (1995) Mitochondrial receptor complex protein. Journal of Biological Chemistry 270, 30571-30575.
    • Brian L. Webb, Michael M. Cox and Ross B. Inman. (1995) An interaction between the Escherichia coli RecF and RecR proteins dependent on ATP and double-stranded DNA. Journal of Biological Chemistry 270, 31397-31404.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB036VM pET-21a-d(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors