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69741 pET-21b(+) DNA - Novagen

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      OverviewThe pET-21a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-24a-d(+) only by their selectable marker (ampicillin vs. kanamycin resistance). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB036). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.

      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to:
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69741
      Brand Family Novagen®
      Product Information
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Supplemental Information


      pET-21b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number


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    • Chung-Hang Leung, et al. (2006) Eriocalyxin B inhibits nuclear factor-B activation by interfering with the binding of both p65 and p50 to the response element in a noncompetitive manner. Molecular Pharmacology 70, 1946-1955.
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    • Zhiyu Li, Hiroshi Hiasa and Russell DiGate. (2005) Bacillus cereus DNA topoisomerase I and IIIα: purification, characterization and complementation of Escherichia coli TopoIII activity. Nucleic Acids Research 33, 5415-5425.
    • Ken Okada and Toshiharu Hase. (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. Journal of Biological Chemistry 280, 60672-20679.
    • Savvas Saouros, et al. (2005) A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell-adhesion complex. Journal of Biological Chemistry 280, 38583-38591.
    • Hideshi Yokoyama and Ikuo Matsui. (2005) A novel thermostable membrane protease forming an operon with a stomatin homolog from hyperthermophilic archaeon Pyrococcus horikoshii. Journal of Biological Chemistry 280, 6588-6594.
    • Zilian Zhang, et al. (2005) Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain7. Journal of Biological Chemistry 280, 9698-9705.
    • Tung-Ju Hsieh, et al. (2004) Structure of the topoisomerase IV C-terminal domain: a broken-propeller implies a role as geometry facilitator in catalysis. Journal of Biological Chemistry 279, 55587-55593.
    • Elizabeth A. Walker, et al. (2001) Functional expression, characterization, and purification of the catalytic domain of human 11-β-hydroxysteroid dehygronase type I. Journal of Biological Chemistry 276, 21343-21350.
    • Mariagrazia Pizza, et al. (2000) Identification of vaccine candidates against serogroup B Meningococcus by whole-genome sequencing. Science 287, 1816-1820.
    • Quinn L. Deveraux, et al. (1998) IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. European Molecular Biology Organization Journal 17, 2215-2223.
    • Doris M. Kraemer, et al. (1995) The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. Journal of Biological Chemistry 270, 19017-19021.
    • Laura M. McMurry and Stuart B. Levy. (1995) The NH2-terminal half of the Tn10-specified tetracycline efflux protein TetA contains a dimerization domain. Journal of Biological Chemistry 270, 22752-22757.
    • User Protocols

      TB055 pET System Manual

      Vector Map

      TB036VM pET-21a-d(+) Vector Map

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      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors