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69663 pET-17b DNA - Novagen

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Replacement Information


Catalog NumberPackaging Qty/Pack
69663-3 Plastic ampoule 10 μg
OverviewThe pET-17b vector carries an N-terminal 11aa T7•Tag® sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker (1). Unique sites (except for the two BstX I sites) are shown on the vector map (TB047). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.

This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to:
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69663
Brand Family Novagen®
References1. Seed, B. (1987) Nature 329, 840.
Product Information
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
69663-3 07790788052614


pET-17b DNA - Novagen Certificates of Analysis

TitleLot Number


Reference overview
1. Seed, B. (1987) Nature 329, 840.


  • Heidi A. Erickson, Michelle D. Jund and Christopher A. Pennell. 2006. Protein Engineering Design and Selection 19, 37.
  • Aichberger, K.J., et al. 2005. Journal of Immunology 175, 1286.
  • Kim, Y., et al. 2005. Journal of Biological Chemistry 280, 23273.
  • Georg W. Mayr, Sabine Windhorst and Kirsten Hillemeier. 2005. Journal of Biological Chemistry 280, 13229.
  • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
  • Suzuki, M., et al. 2005. Journal of Biological Chemistry 280, 21444.
  • Zhao, B., et al. 2005. Journal of Biological Chemistry 280, 11599.
  • James S. Parker, S. Mark Roe and David Barford. 2004. European Molecular Biology Organization Journal 23, 4727.
  • M. Schuster, M. L. Urbanowski and E. P. Greenberg. 2004. Procedings of the National Academy of Science 101, 15833.
  • Mazumder, B., et al. 2003. Cell 115, 187.
  • Shi, Z-Z., et al. 1996. Nature Genetics 14, 361.
  • User Protocols

    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    TB047VM pET-17b Vector Map

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