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71330 pCDF-1b DNA - Novagen

71330
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pCDF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag that results in native protein after purification and cleavage. This plasmid carries an origin derived from CloDF13 (1) and streptomycin/spectinomycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors.

      The vector contains a T7 promoter, lac operator, ribosome binding site (rbs), an amino terminal His•Tag® coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector-encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many of other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (2).
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number71330
      Brand Family Novagen®
      References
      References1. Nijkamp, H.J.J., De Lang, R., Stuitje, A.R., Van Den Elzen P.J.M., Veltkamp, E., and Van Putten, A.J. (1986) Plasmid 16, 135–160.

      2. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
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      Product Usage Statements
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      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
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      Supplemental Information
      Specifications

      Documentation

      pCDF-1b DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pCDF-1b DNA - Novagen Certificates of Analysis

      TitleLot Number
      71330

      References

      Reference overview
      1. Nijkamp, H.J.J., De Lang, R., Stuitje, A.R., Van Den Elzen P.J.M., Veltkamp, E., and Van Putten, A.J. (1986) Plasmid 16, 135–160.

      2. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

      Citations

      Title
    • Michael A. Fischbach, et al. (2007) Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Procedings of the National Academy of Science 104, 11951-11956.
    • Catherine E. Vrentas, et al. (2005) Response of RNA polymerase to ppGpp: requirement for the {omega} subunit and relief of this requirement by DksA. Genes and Development 19, 2378-2387.
    • Christine N. Zanghi, et al. (2005) A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda. Nucleic Acids Research 33, e160.
    • User Protocols

      Title
      TB055 pET System Manual
      TB401 pRSF and pCDF Vectors

      Vector Map

      Title
      TB392 pCDF-1b Vector Map

      Vector Sequence

      Title
      pCDF-1b Vector Sequence