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70045 pBAC™4x-1 DNA - Novagen

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Catalog NumberPackaging Qty/Pack
70045-3 Plastic ampoule 10 μg
OverviewpBAC4x-1 is a baculovirus transfer plasmid designed for cloning and co-expression of up to four target genes or multiple copies of the same gene in insect cells. The plasmid contains two polh promoters and two p10 promoters, each of which is upstream of unique cloning sites for sequential insertion of target genes. The homologous promoters are in opposite orientations to minimize recombination. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the promoters. The vector provides optional expression of a C-terminal His•Tag® fusion sequence from the polh right-hand promoter by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the clockwise promoters is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to this strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.

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Catalogue Number70045
Brand Family Novagen®
Product Information
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
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Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
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pBAC™4x-1 DNA - Novagen SDS


Safety Data Sheet (SDS) 

pBAC™4x-1 DNA - Novagen Certificates of Analysis

TitleLot Number


  • Junmin Peng, Nick F. Marshall and David H. Price. (1998) Identification of a cyclin subunit required for the function of Drosophila P-TEFb. Journal of Biological Chemistry 273, 13855-13860.
  • Vector Map

    TB133 pBAC4x-1 Vector Map

    Vector Sequence

    pBAC4x-1 Sequence