509887 miR-372/3 Inhibitor, Neomycin-S - Calbiochem

509887
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₃₉H₆₀O₁₄N₁₂S

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      Glass bottle 2 mg
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      Description
      OverviewA cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).

      Please note that the molecular weight for this compound is batch-specific due to variable water content.
      Catalogue Number509887
      Brand Family Calbiochem®
      SynonymsmiRNA-372 Inhibitor, miRNA-373 Inhibitor
      References
      ReferencesVo, D.D., et al. 2014. ACS Chem. Biol. 9, 711.
      Product Information
      FormOff-white solid
      Hill FormulaC₃₉H₆₀O₁₄N₁₂S
      Chemical formulaC₃₉H₆₀O₁₄N₁₂S
      Hygroscopic Hygroscopic
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetmiRNA-272/273
      Purity≥98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      miR-372/3 Inhibitor, Neomycin-S - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Vo, D.D., et al. 2014. ACS Chem. Biol. 9, 711.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-June-2014 JSW
      SynonymsmiRNA-372 Inhibitor, miRNA-373 Inhibitor
      DescriptionA cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).
      FormOff-white solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₃₉H₆₀O₁₄N₁₂S
      Purity≥98% by HPLC
      SolubilityDMSO (100 mg/ml)
      Storage ≤ -70°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesVo, D.D., et al. 2014. ACS Chem. Biol. 9, 711.