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324892 eIF2-GTP-tRNAiMet ternary complex Inhibitor, BTdCPU - CAS 1257423-87-2 - Calbiochem

The eIF2-GTP-tRNAiMet ternary complex Inhibitor, BTdCPU, also referenced under CAS 1257423-87-2, controls the biological activity of eIF2-GTP-tRNAiMet ternary complex.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: heme-regulated inhibitor kinase activator, BTdCPU, BTdCPU, 1-(benzo[d][1,2,3]thiadiazol-6-yl)-3-(3,4-dichlorophenyl)urea, N,N'-diarylurea;translation initiation tenrary complex inhibitor

324892
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
1257423-87-2C₁₃H₈Cl₂N₄OS

Products

Catalog NumberPackaging Qty/Pack
324892-10MG Glass bottle 10 mg
Description
OverviewA cell-permeable N, N'-diarylurea that acts as an inhibitor of the formation of the ternary complex of eukaryotic translation initiation factor 2 (eIF2), GTP and the initiator methionine tRNA (Met-tRNAi), a key regulatory step of translation initiation. It directly activates heme-regulated inhibitor kinase (HRI), which then phosphorylates eIF2α in cells at 10 µM and in cell-free extracts dose-dependently, and thereby prevents respective ternary complex formation without elevating oxidative stress. The sensitivities of various cancer cell lines to the anti-proliferative effects of this compound are shown to correlate with the expression of HRI. Furthermore, this compound displays tumor growth inhibition in a mice xenograft model of MCF-7 human breast cancer cells at 175 mg/kg, in vivo.
Catalogue Number324892
Brand Family Calbiochem®
Synonymsheme-regulated inhibitor kinase activator, BTdCPU, BTdCPU, 1-(benzo[d][1,2,3]thiadiazol-6-yl)-3-(3,4-dichlorophenyl)urea, N,N'-diarylurea;translation initiation tenrary complex inhibitor
References
ReferencesChen, T., et al. 2011. Nat. Chem. Biol. 7, 610.
Product Information
CAS number1257423-87-2
FormWhite solid
Hill FormulaC₁₃H₈Cl₂N₄OS
Chemical formulaC₁₃H₈Cl₂N₄OS
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥99% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

eIF2-GTP-tRNAiMet ternary complex Inhibitor, BTdCPU - CAS 1257423-87-2 - Calbiochem Certificates of Analysis

TitleLot Number
324892

References

Reference overview
Chen, T., et al. 2011. Nat. Chem. Biol. 7, 610.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-June-2012 JSW
Synonymsheme-regulated inhibitor kinase activator, BTdCPU, BTdCPU, 1-(benzo[d][1,2,3]thiadiazol-6-yl)-3-(3,4-dichlorophenyl)urea, N,N'-diarylurea;translation initiation tenrary complex inhibitor
DescriptionA cell-permeable N,N'-diarylurea that acts as an inhibitor of the formation of the ternary complex of eukaryotic translation initiation factor 2 (eIF2), GTP and the initiator methionine tRNA (Met-tRNAi), a key regulatory step of translation initiation. It directly activates heme-regulated inhibitor kinase (HRI), which then phosphorylates eIF2α in cells at 10 µM and in cell-free extracts dose-dependently, and thereby prevents respective ternary complex formation without elevating oxidative stress. The sensitivities of various cancer cell lines to the anti-proliferative effects of this compound are shown to correlate with the expression of HRI. Furthermore, this compound displays tumor growth inhibition in a mice xenograft model of MCF-7 human breast cancer cells at 175 mg/kg, in vivo.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number1257423-87-2
Chemical formulaC₁₃H₈Cl₂N₄OS
Structure formulaStructure formula
Purity≥99% by HPLC
SolubilityDMSO (25 mg/ml; clear, colorless solution)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChen, T., et al. 2011. Nat. Chem. Biol. 7, 610.