Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

530538 cPLA2α Inhibitor II, Pyrrophenone - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₄₉H₃₇F₂N₃O₅S₂

Products

Catalog NumberPackaging Qty/Pack
5.30538.0001 Glass bottle 1 mg
Description
OverviewA cell-permeable, non-toxic, pyrolidine derivative that acts as a highly potent, and reversible inhibitor of cytosolic phospholipase A2a (cPLA2a; IC50 = 4.2 nM). Does not inhibit the activities of PLA IB and IIA groups even at higher concentrations (~250 µM). Unlike AACOCF3 (Cat. No. 100109), its action is shown to be rapid and does not require a pre-incubation period. Diminishes the release of arachidonic acid (IC50 = 24 nM), prostaglandin E2 (PGE2; IC50 = 25 nM) and leukotriene C4 (IC50 = 14 nM) in A23187 stimulated THP-1 cells. Its effects on production of PGE2 and thromboxane B2 are comparable to those of indomethacin. Also shown to inhibit the lysophospholipase and esterase activity of cPLA2&alpha.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number530538
Brand Family Calbiochem®
SynonymsPhospholipase A2α Inhibitor
References
ReferencesOno, T., et al. 2002. Biochem. J. 363, 727.
Seno, K., et al. 2001. Bioorg. Med. Chem. Lett. 11, 587.
Product Information
FormOff-white powder
Hill FormulaC₄₉H₃₇F₂N₃O₅S₂
Chemical formulaC₄₉H₃₇F₂N₃O₅S₂
Quality LevelMQ100
Applications
Biological Information
Primary TargetPLA2a
Primary Target IC<sub>50</sub>4.2 nM
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.30538.0001 04055977260892

Documentation

cPLA2α Inhibitor II, Pyrrophenone - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Ono, T., et al. 2002. Biochem. J. 363, 727.
Seno, K., et al. 2001. Bioorg. Med. Chem. Lett. 11, 587.

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-October-2014 JSW
SynonymsPhospholipase A2α Inhibitor
DescriptionA cell-permeable, non-toxic, pyrolidine derivative that acts as a highly potent, and reversible inhibitor of cytosolic phospholipase A2a (cPLA2a; IC50 = 4.2 nM). Does not inhibit the activities of PLA IB and IIA groups even at higher concentrations (~250 µM). Unlike AACOCF3 (Cat. No. 100109), its action is shown to be rapid and does not require a pre-incubation period. Diminishes the release of arachidonic acid (IC50 = 24 nM), prostaglandin E2 (PGE2; IC50 = 25 nM) and leukotriene C4 (IC50 = 14 nM) in A23187 stimulated THP-1 cells. Its effects on production of PGE2 and thromboxane B2 are comparable to those of indomethacin. Also shown to inhibit the lysophospholipase and esterase activity of cPLA2&alpha.
FormOff-white powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₄₉H₃₇F₂N₃O₅S₂
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesOno, T., et al. 2002. Biochem. J. 363, 727.
Seno, K., et al. 2001. Bioorg. Med. Chem. Lett. 11, 587.