GF094 Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform

GF094
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT Human E.coli Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of VEGF
      Description
      Catalogue NumberGF094
      ReplacesGF025
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionVascular Endothelial Growth Factor, Recombinant human, 165aa isoform
      OverviewVascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.
      Alternate Names
      • VEGF
      References
      Product Information
      PresentationLyophilized from sterile filtered 20 mM acetic acid, containing no additives.
      Quality LevelMQ100
      Applications
      ApplicationVascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, human VEGF exerts its biological activity in the concentration range of 0.1 to 50.0 ng/mL. Responding cells are (partial list): Endothelial cells.
      Biological Information
      PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of VEGF
      SourceE.coli
      Specific ActivityThe biological activity of Millipore's human VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC). Mitogenic activity was obtained in a concentration range of 1.0-8.0 ng/mL.
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
      Gene Symbol
      • VEGFA
      • MGC70609
      • VPF
      • VEGF
      • VEGF-A
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
      SIZE: 232 amino acids; 27042 Da
      SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
      SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
      TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
      SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.
      Stem Cell Type
      • Neural Stem Cells
      • Mesenchymal Stem Cells
      • Hematopoietic Stem Cells
      Molecular Weight38.2 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. Reconstituted material can be stored at 2°C-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform SDS

      Title

      Safety Data Sheet (SDS) 

      Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform Certificates of Analysis

      TitleLot Number
      VEGF165aa, HUMAN RECOMBINANT PROTEIN3006675
      VEGF165aa, HUMAN RECOMBINANT PROTEIN2823180
      VEGF165aa, HUMAN RECOMBINANT PROTEIN2944653
      VEGF165aa, HUMAN RECOMBINANT PROTEIN - 20731842073184
      VEGF165aa, HUMAN RECOMBINANT PROTEIN - 22816712281671
      VEGF165aa, HUMAN RECOMBINANT PROTEIN - DAM1732869DAM1732869
      VEGF165aa, HUMAN RECOMBINANT PROTEIN -25322502532250
      VEGF165aa, HUMAN RECOMBINANT PROTEIN -25576232557623

      Data Sheet

      Title
      VEGF165aa, HUMAN RECOMBINANT PROTEIN

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 3

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors