110275 VRBD (Violet Red Bile Dextrose) agar

110275
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Overview

Replacement Information

Key Specifications Table

ph ValueSolubility
7.3 (40 g/l, H₂O, 37 °C) (after autoclaving) 40 g/l

Pricing & Availability

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1102750500
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      Plastic bottle 500 g
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      Description
      Catalogue Number110275
      SynonymsCrystal-violet neutral-red bile dextrose agar, VRBD-agar (Crystal-violet neutral-red bile dextrose agar) acc. to MOSSEL
      OverviewGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
      References
      Product Information
      HS Code3821 00 00
      Quality LevelMQ300
      Applications
      Biological Information
      Physicochemical Information
      pH value7.3 (40 g/l, H₂O, 37 °C) (after autoclaving)
      Bulk density720 kg/m3
      Solubility40 g/l
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Storage class10 - 13 Other liquids and solids
      WGKWGK 1 slightly hazardous to water
      Disposal3
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Appearance (clearness)clear
      Appearance (color)red
      Solidification behaviour (4 hrs., 45 °C)liquid
      pH-value (25 °C)7.2 - 7.6
      Typical composition (g/litre): Peptone from gelatine 7.0; Yeast extract 3.0; Sodium chloride 5.0; D(+)Glucose 10.0; Bile salt mixture 1.5; Neutralred 0.03; Crystal violet 0.002; Agar-agar 13.0.
      Growth promotion test in accordance with the current version of harmonised method of EP, USP and JP.
      Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
      10 - 100
      Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))10 - 100
      Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032))≥ 10000
      Inoculum (Bacillus cereus ATCC 11778 (WDCM 00001))≥ 10000
      Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
      Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
      Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))
      50 - 150 %
      Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))50 - 150 %
      Colony color (Escherichia coli ATCC 8739 (WDCM 00012))red colonies
      Colony color (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))nearly colourless to slightly red colonies
      Precipitate (Escherichia coli ATCC 8739 (WDCM 00012))+
      Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032))no growth
      Growth (Bacillus cereus ATCC 11778 (WDCM 00001))no growth
      Incubation:
      up to 18 hours at 30-35°C aerobic
      Staphylococcus aureus and Bacillus cereus at least 24 hours.
      Reference medium: Tryptic Soy Agar
      Growth promotion test in accordance with the current version of DIN EN ISO 11133.
      Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
      Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
      Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))
      Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))
      Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
      Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
      Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031))
      Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030))
      Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))
      ≥ 50 %
      Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 50 %
      Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))≥ 50 %
      Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))≥ 50 %
      Colonies (Escherichia coli ATCC 8739 (WDCM 00012))Pink to red colonies with or without precipitation halo
      Colonies (Escherichia coli ATCC 25922 (WDCM 00013))Pink to red colonies with or without precipitation halo
      Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031))Pink to red colonies with or without precipitation halo
      Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030))Pink to red colonies with or without precipitation halo
      Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))total inhibition
      Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))total inhibition
      Incubation: 24 ± 2 hours at 37 ± 1 °C
      A recovery rate of 50 % is equivalent to a productivity value of 0.5.
      The indicated colony counts result from the sum of a triple determination.
      Reference medium: Tryptic Soy Agar
      The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

      Documentation

      VRBD (Violet Red Bile Dextrose) agar SDS

      Title

      Safety Data Sheet (SDS) 

      VRBD (Violet Red Bile Dextrose) agar Certificates of Analysis

      Product Number PackagingSpecificationLot Number
      1102750500 Plastic bottlePDF Specification Document

      Certificates

      Title

      Brochure

      Title
      A sweet solution for safety. Granulated media (MilliporeSigma)

      Data Sheet

      Title
      Technical Data Sheet 1.10275.0500 GranuCult™ VRBD (Violet Red Bile Dextrose) Agar acc. EP, USP, JP and ISO 21528 (MilliporeSigma)

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      Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media
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      Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Coliforms, E. coli & Enterobacteriaceae
      Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Listeria
      Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > E. Sakazakii
      Industrial Microbiology > Bioburden Testing > Bioburden Testing for Pharmaceutical & Cosmetics Industry > Test for specified Microorganisms
      Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > E. Coli O157 & STEC
      Industrial Microbiology > Culture Media > Culture Media for Pharmaceutical & Cosmetics Industry > Dehydrated Culture Media > Dilution, Enrichment & Microbial Enumeration > Environmental Tests