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662065 Ubiquitin, Methylated, Human, Recombinant

662065
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662065-1MG
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      Description
      OverviewRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
      Catalogue Number662065
      Brand Family Calbiochem®
      SynonymsMe-Ub
      References
      ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
      Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
      Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
      Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
      Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
      Product Information
      FormLyophilized solid
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
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      Supplemental Information
      Specifications

      Documentation

      Ubiquitin, Methylated, Human, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Ubiquitin, Methylated, Human, Recombinant Certificates of Analysis

      TitleLot Number
      662065

      References

      Reference overview
      Hershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
      Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
      Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
      Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
      Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsMe-Ub
      DescriptionRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
      FormLyophilized solid
      Recommended reaction conditionsTypical concentrations for non-rate-limiting support of in vitro conjugation reactions range from 200 µM to 1 mM depending on the reaction conditions.
      Purity≥95% by SDS-PAGE
      SolubilityAqueous buffers (50 mg/ml)
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
      Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
      Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
      Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
      Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.