GF023 Tumor Necrosis Factor-α Protein, Recombinant human

GF023
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT Human E.coli Greater than 97% by SDS-PAGE
      Description
      Catalogue NumberGF023
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionTumor Necrosis Factor-α Protein, Recombinant human
      OverviewTumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. GF023 is a recombinant polypeptide containing the mature form of human TNF-a with a polyhistidine tag at its amino terminal.
      Alternate Names
      • TNF-alpha
      References
      Product Information
      PresentationLyophilized from 3mM Tris, pH 8.0.
      Quality LevelMQ100
      Applications
      ApplicationTumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/mL. Responding cells are (partial list): Tumor cells, certain other cells.
      Biological Information
      PurityGreater than 97% by SDS-PAGE
      SourceE.coli
      Specific ActivityChemicon's human TNF-alpha, is fully biologically active when compared to standards. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin-D is <= 0.05 ng/mL corresponding to a specific activity of >= 2 x 107 units/mg.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
      Gene Symbol
      • TNF
      • TNFSF2
      • Cachectin
      • cachectin
      • DIF
      • TNF-alpha
      • OTTHUMP00000037669
      • TNFA
      • TNF-a
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
      SIZE: 233 amino acids; 25644 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
      SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
      Stem Cell Type
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with sterile distilled water to a concentration of 0.1-1 mg/mL. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. This solution can be diluted into other buffered solutions and stored at 4°C for one week or -20°C for future use. Reconstituted TNF-alpha should be stored in undiluted aliquots at -20°C for up to six months.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Tumor Necrosis Factor-α Protein, Recombinant human SDS

      Title

      Safety Data Sheet (SDS) 

      Tumor Necrosis Factor-α Protein, Recombinant human Certificates of Analysis

      TitleLot Number
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-a - 21677962167796
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-a - 23090452309045
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-a -26825242682524
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α2452491
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α2946036
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α2846655
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α - 23665552366555
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α - 23905052390505
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α - 24911912491191
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-α - 25299862529986

      Data Sheet

      Title
      RECOMBINANT HUMAN TUMOR NECROSIS FACTOR-alpha

      Related Products & Applications

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors