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65035 Trypsin Inhibitor, Soybean - CAS 9035-81-8 - Calbiochem

65035
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Overview

Replacement Information

Key Specifications Table

CAS #
9035-81-8

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65035-100MG
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      Plastic ampoule 100 mg
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      65035-1GM
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          Description
          OverviewA reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, pH-dependent manner (Kd = 500 nM at pH 7.8).
          Catalogue Number65035
          Brand Family Calbiochem®
          References
          ReferencesLilly, C.M., et al. 1993. J. Clin. Invest. 91, 235.
          Uchino, T., et al. 1993. J. Biol. Chem. 268, 527.
          Birk, Y. 1976. Methods Enyzmol. 45, 700.
          Product Information
          CAS number9035-81-8
          Unit of DefinitionOne unit is defined as the amount of protein that will inhibit 1 unit of trypsin activity using BAEE as a substrate at 25°C, pH 7.6.
          ATP CompetitiveN
          FormOff-white lyophilized solid
          Hygroscopic Hygroscopic
          ReversibleY
          Quality LevelMQ100
          Applications
          ApplicationTrypsin Inhibitor, Soybean, CAS 9035-81-8, is a reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, pH-dependent manner (Kd = 500 nM at pH 7.8).
          Biological Information
          Primary Targettrypsin
          Primary Target IC<sub>50</sub>Kd = 500 nM at pH 7.8 against trypsin
          Specific Activity≥4000 units/mg protein
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Trypsin Inhibitor, Soybean - CAS 9035-81-8 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Trypsin Inhibitor, Soybean - CAS 9035-81-8 - Calbiochem Certificates of Analysis

          TitleLot Number
          65035

          References

          Reference overview
          Lilly, C.M., et al. 1993. J. Clin. Invest. 91, 235.
          Uchino, T., et al. 1993. J. Biol. Chem. 268, 527.
          Birk, Y. 1976. Methods Enyzmol. 45, 700.

          Citations

          Title
        • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2017 JSW
          DescriptionReversible inhibitor of trypsin that binds to trypsin in a stoichiometric, reversible and pH-dependent manner (Kd = 0.5 nM at pH 7.8). Also inhibits, to a lesser degree, chymotrypsin, kallikrein, plasmin, and other serine proteases.
          FormOff-white lyophilized solid
          Recommended reaction conditionsInactivates trypsin on an equal molar basis. To ensure complete inhibition of trypsin activity, a working concentration of soybean trypsin inhibitor should exceed that of trypsin by a factor of at least 2.
          CAS number9035-81-8
          Specific activity≥4000 units/mg protein
          Unit definitionOne unit is defined as the amount of protein that will inhibit 1 unit of trypsin activity using BAEE as a substrate at 25°C, pH 7.6.
          Solubility67 mM potassium phosphate, pH 7.6 (5 mg/ml)
          Storage -20°C
          Hygroscopic
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C.
          Toxicity Standard Handling
          ReferencesLilly, C.M., et al. 1993. J. Clin. Invest. 91, 235.
          Uchino, T., et al. 1993. J. Biol. Chem. 268, 527.
          Birk, Y. 1976. Methods Enyzmol. 45, 700.
          Citation
        • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.