616377 Toxin B, Clostridium difficile - Calbiochem

616377
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Key Specifications Table

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616377-2UG
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      Plastic ampoule 2 μg
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      Description
      OverviewA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositiol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
      Catalogue Number616377
      Brand Family Calbiochem®
      SynonymsCytotoxin
      References
      ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
      Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
      Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
      Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
      Product Information
      ATP CompetitiveN
      FormLyophilized
      FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityCytotoxic as determined in a cell rounding assay using human foreskin cells.
      Primary TargetSmall GTPases, such as Rho, Rac, and Cdc42
      Puritymajor band at >200 kDa
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXW5807300
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 36-26

      Wear suitable protective clothing.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Toxin B, Clostridium difficile - Calbiochem Certificates of Analysis

      TitleLot Number
      616377

      References

      Reference overview
      Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
      Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
      Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
      Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-April-2009 JSW
      SynonymsCytotoxin
      DescriptionA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
      FormLyophilized
      FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
      RTECSXW5807300
      Puritymajor band at >200 kDa
      Biological activityCytotoxic as determined in a cell rounding assay using human foreskin cells.
      SolubilitySterile dH₂O. Please see vial label for lot-specific reconstitution volume.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
      Toxicity Irritant
      ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
      Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
      Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
      Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.