605204 Thrombin, Immobilized, Human Plasma

605204
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605204-1ML
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      Glass bottle 1 ml
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      Description
      OverviewNative thrombin from human plasma, bound to agarose beads.
      Catalogue Number605204
      Brand Family Calbiochem®
      References
      ReferencesXiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.
      Product Information
      FormSuspension
      FormulationIn 100 mM NaCl, 20 mM Tris-HCl, ≤0.1% sodium azide, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Thrombin, Immobilized, Human Plasma SDS

      Title

      Safety Data Sheet (SDS) 

      Thrombin, Immobilized, Human Plasma Certificates of Analysis

      TitleLot Number
      605204

      References

      Reference overview
      Xiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-May-2008 RFH
      DescriptionNative thrombin from human plasma, bound to agarose beads. Thrombin is a key enzyme in the coagulation cascade. Converts fibrinogen to fibrin and activates factor XIII, which cross-links and stabilizes the fibrin polymer.
      FormSuspension
      FormulationIn 100 mM NaCl, 20 mM Tris-HCl, ≤0.1% sodium azide, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Guidelines for Fusion Protein Cleavage: Adapted from Xiang, et al (see references) Please note that this protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. 1. Immobilized thrombin can be used either with a column or in batch format. For use with batch method the gel may be centrifuged at 3000 rpm or less for separation of agarose from supernatant containing cleaved protein. Higher speeds will result in difficulty resuspending the agarose. 2. A rule of thumb wt/wt ratio to obtain efficient cleavage for most fusion proteins is 1:10 to 1:100 (thrombin:fusion protein) 3. Determine the amount of thrombin required to achieve desired cleavage. Add buffer to this amount of immobilized thrombin to form a slurry. Acceptable buffers include Tris-HCl, containing 150 mM NaCl, citrate buffer, or PBS; the optimal pH is 7.4. For column cleavage, wash the column 2–3 times with buffer prior to addition of sample. The protein should also be resuspended in the same buffer prior to cleavage. 4. Incubation: 25°C, generally overnight; time course including 2 h, 5 h, and 18 h is recommended; assess cleavage by SDS-PAGE. It may be necessary to check cleavage at intermittent intervals (e.g. every 30 min.). 5. For column cleavage, protein that migrates through the column may be monitored at 280 nm.
      SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      Merck USA index14 9383
      ReferencesXiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.