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532177 Tdp1 Inhibitor, CD00509 - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₉H₆N₂O₃S

Products

Catalog NumberPackaging Qty/Pack
5321770001 Glass bottle 25 mg
Description
OverviewA cell permeable alkylidene barbiturate analog that acts as a potent and specific inhibitor of tyrosyl-DNA phosphodiesterase 1 (Tdp1; IC50 = 710 nM) and increases the number of g-H2AX foci in MCF-7 cells. Shown to sensitize MCF-7 cells to camptothecin (CPT), increase DNA breaks, and reduce cell proliferation. However, it does not affect the viability of normal mammary epithelial cells up to 10 µM levels. Synergistically reduces cell proliferation and induces apoptosis in MCF-7 cells when combined with Rucaparib, a PARP-1 inhibitor.
Catalogue Number532177
Brand Family Calbiochem®
SynonymsTyrosyl-DNA phosphodiesterase 1 Inhibitor
References
ReferencesDean, R.A., et al. 2014. J. Biolmol. Screen. 19, 1372.
Product Information
FormYellow powder
Hill FormulaC₉H₆N₂O₃S
Chemical formulaC₉H₆N₂O₃S
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetTdp1
Primary Target IC<sub>50</sub>710 nM
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5321770001 04055977287172

Documentation

Tdp1 Inhibitor, CD00509 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Dean, R.A., et al. 2014. J. Biolmol. Screen. 19, 1372.

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-January-2015 JSW
SynonymsTyrosyl-DNA phosphodiesterase 1 Inhibitor
DescriptionA cell permeable alkylidene barbiturate analog that acts as a potent and specific inhibitor of tyrosyl-DNA phosphodiesterase 1 (Tdp1; IC50 = 710 nM) and increases the number of g-H2AX foci in MCF-7 cells. Shown to sensitize MCF-7 cells to camptothecin (CPT), increase DNA breaks, and reduce cell proliferation. However, it does not affect the viability of normal mammary epithelial cells up to 10 µM levels. Synergistically reduces cell proliferation and induces apoptosis in MCF-7 cells when combined with Rucaparib, a PARP-1 inhibitor.
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₉H₆N₂O₃S
Purity≥97% by HPLC
SolubilityDMSO
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesDean, R.A., et al. 2014. J. Biolmol. Screen. 19, 1372.