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S7701 TRAPeze® Telomerase Positive Control Cell Pellet

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S7701
1 vial  1x10(e6) cells
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Overview

Replacement Information
Description
Catalogue NumberS7701
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionTRAPeze® Telomerase Positive Control Cell Pellet
OverviewThis product is intended for use with the components of the TRAPEZE® Telomerase Detection Kit (Catalog # S7700), the TRAPEZE® XL Telomerase Detection Kit (Catalog # S7707) and the TRAPEZE® ELISA Telomerase Detection Kit (Catalog # S7750). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested.



Material Provided

Each vial of S7701 TRAPEZE® Positive Control Cell Pellet contains one million cells, pelletted to the bottom of the microcentrifuge tube.
References
Product Information
Quality LevelMQ100
Applications
ApplicationThis product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit.
Application NotesInstructions for Use

1. Resuspend the cell pellet in 200 μl of 1X CHAPS Lysis Buffer.

2. Incubate the suspension on ice for 30 minutes.

3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C.

4. Transfer 160 μl of the supernatant into a fresh tube.

5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination.

6. Use 1000 cell equivalents (2 μl of a 1:10 dilution) per TRAP assay, according to the kit manuals.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMust be stored at -75°C to -85°C. Telomerase in specimens stored as a cell pellet is stable at this temperature for several years. Once the cell extract is made (in 1X CHAPS Lysis Buffer), it is stable for 1 year, if not subjected to more than 5 freeze-thaw cycles
Packaging Information
Material Size1 vial
Material Package1x10(e6) cells
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
S7701 04053252316456

Documentation

TRAPeze® Telomerase Positive Control Cell Pellet SDS

Title

Safety Data Sheet (SDS) 

TRAPeze® Telomerase Positive Control Cell Pellet Certificates of Analysis

TitleLot Number
Telomerase Positive Control 2868357
Telomerase Positive Control 2957631
Telomerase Positive Control 3013308
Telomerase Positive Control 2833526
Telomerase Positive Control - 2392288 2392288
Telomerase Positive Control - 2064552 2064552
Telomerase Positive Control - 2200944 2200944
Telomerase Positive Control - 2206396 2206396
Telomerase Positive Control - 2283339 2283339
Telomerase Positive Control - 2533221 2533221

References

Reference overviewPub Med ID
Inhibition of telomerase by G-quartet DNA structures.
A M Zahler,J R Williamson,T R Cech,D M Prescott
Nature  350  1991

Show Abstract
2023635 2023635