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S7700 TRAPeze® Telomerase Detection Kit

S7700
112 assays  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsDetection Methods
MaRT-PCRChromogenic
Description
Catalogue NumberS7700
Brand Family Chemicon®
Trade Name
  • TRAPeze
  • Chemicon
DescriptionTRAPeze® Telomerase Detection Kit
OverviewThe TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.



Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested.
Alternate Names
  • TRAP Assay
Background InformationTelomeres are specific structures found at the ends of chromosomes in
eukaryotes. In human chromosomes, telomeres consist of thousands of copies of 6 base repeats (TTAGGG). It has been suggested that telomeres protect chromosome ends, because damaged chromosomes lacking telomeres undergo fusion, rearrangement and translocation. In somatic cells, telomere length is progressively shortened with each cell division both in vivo and in vitro, due to the inability of the DNA polymerase complex to replicate the very 5' endof the lagging strand .
Telomerase is a ribonucleoprotein that synthesizes and directs the telomeric
repeats onto the 3' end of existing telomeres using its RNA component as a
template. Telomerase activity has been shown to be specifically expressed in immortal cells, cancer and germ cells, where it compensates for telomere shortening during DNA replication and thus stabilizes telomere length. These observations have led to a hypothesis that telomere length may function as a “mitotic clock” to sense cell aging and eventually signal replicative senescence or programmed cell death. Therefore, expression of telomerase activity in cancer cells may be a necessary and essential step for tumor development and progression. The causal relationship between expression of telomerase and telomere length stabilization and the extension of the life span of the human cell has recently been reported.
References
Product Information
Detection methodChromogenic
Quality LevelMQ100
Applications
ApplicationA highly sensitive in vitro PCR based assay for detecting telomerase activity based on the TRAP assay (Telomeric Repeat Amplification Protocol).
Key Applications
  • PCR
Biological Information
Species Reactivity
  • Mammals
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size112 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
S7700 04053252014062

Documentation

TRAPeze® Telomerase Detection Kit SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewApplicationSpeciesPub Med ID
Pattern of Cell Proliferation During Budding in the Colonial Ascidian Diplosoma listerianum.
Sköld HN, Stach T, Bishop JD, Herbst E, Thorndyke MC
The Biological bulletin  221  126-36.  2011

Show Abstract
21876115 21876115
Detection of telomerase activity in high concentration of cell lysates using primer-modified gold nanoparticles.
Yi Xiao,Karen Y Dane,Takanori Uzawa,Andrew Csordas,Jiangrong Qian,H Tom Soh,Patrick S Daugherty,Eric T Lagally,Alan J Heeger,Kevin W Plaxco
Journal of the American Chemical Society  132  2010

Show Abstract
20932008 20932008
3D nuclear organization of telomeres in the Hodgkin cell lines U-HO1 and U-HO1-PTPN1: PTPN1 expression prevents the formation of very short telomeres including t-stumps.
Hans Knecht,Silke Brüderlein,Silke Wegener,Daniel Lichtensztejn,Zelda Lichtensztejn,Bruno Lemieux,Peter Möller,Sabine Mai
BMC cell biology  11  2010

Show Abstract Full Text Article
21144060 21144060
Establishment and characterization of a human retinal pericyte line: a novel tool for the study of diabetic retinopathy.
Berrone, Elena, et al.
Int. J. Mol. Med., 23: 373-8 (2009)  2009

Show Abstract
ImmunofluorescenceHuman19212656 19212656
Hypomethylation of subtelomeric regions in ICF syndrome is associated with abnormally short telomeres and enhanced transcription from telomeric regions.
Shiran Yehezkel, Yardena Segev, Evani Viegas-Péquignot, Karl Skorecki, Sara Selig
Human molecular genetics  17  2776-89  2008

Show Abstract
18558631 18558631
Evaluation of telomere length maintenance mechanisms in adrenocortical carcinoma.
Tobias Else,Thomas J Giordano,Gary D Hammer
The Journal of clinical endocrinology and metabolism  93  2008

Show Abstract
Human18198226 18198226
Knockdown of p53 combined with expression of the catalytic subunit of telomerase is sufficient to immortalize primary human ovarian surface epithelial cells.
Gong Yang, Daniel G Rosen, Imelda Mercado-Uribe, Justin A Colacino, Gordon B Mills, Robert C Bast, Chenyi Zhou, Jinsong Liu
Carcinogenesis  28  174-82  2007

Show Abstract
16829690 16829690
Telomere dynamics in macaques and humans.
Jeffrey P Gardner, Masayuki Kimura, Weihang Chai, Jameel F Durrani, Levon Tchakmakjian, Xiaojian Cao, Xiaobin Lu, Guanghui Li, Athanasios P Peppas, Joan Skurnick, Woodring E Wright, Jerry W Shay, Abraham Aviv
The journals of gerontology. Series A, Biological sciences and medical sciences  62  367-74  2007

Show Abstract
17452729 17452729
Cationic corrole derivatives: a new family of G-quadruplex inducing and stabilizing ligands.
Boqiao Fu,Jing Huang,Lige Ren,Xiaocheng Weng,Yangyang Zhou,Yuhao Du,Xiaojun Wu,Xiang Zhou,Guangfu Yang
Chemical communications (Cambridge, England)  2007

Show Abstract
17668095 17668095
Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals.
Sunday O Akintoye, Thanh Lam, Songtao Shi, Jaime Brahim, Michael T Collins, Pamela G Robey
Bone  38  758-68  2006

Show Abstract
16403496 16403496

Brochure

Title
Hallmarks of Aging

User Guides

Title
TRAPeze® Telomerase Detection Kit