Key Specifications Table
|CULT||Human||Recombinant human TNFalpha, expressed in E. coli|
|Description||TNFα Protein, recombinant|
|Presentation||Lypholized from 3mM Tris, pH 8.0.|
|Application||The recombinant TNFα Protein is available in a 10 µg format.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Material Size||10 µg|
|Reference overview||Application||Pub Med ID|
|Interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor alpha (TNF alpha) regulate insulin-like growth factor binding protein-1 (IGFBP-1) levels and mRNA abundance in vivo and in vitro|
Benbassat, C. A., et al
Horm Metab Res, 31:209-15 (1999) 1999
|Tumor necrosis factor alpha (TNFalpha) promotes growth of virulent Mycobacterium tuberculosis in human monocytes iron-mediated growth suppression is correlated with decreased release of TNFalpha from iron-treated infected monocytes|
Byrd, T. F.
J Clin Invest, 99:2518-29 (1997) 1997
|Co-regulation and physical association of the 67-kDa monomeric laminin receptor and the alpha6beta4 integrin|
Ardini, E., et al
J Biol Chem, 272:2342-5 (1997) 1997
|Molecular, structural, and biological characteristics of the tumor necrosis factor ligand superfamily.|
Gruss, H J
Int. J. Clin. Lab. Res., 26: 143-59 (1996) 1996
The tumor necrosis factor receptor superfamily at present consists of ten different transmembrane (type I) glycoproteins with characteristic limited sequence homology for the cysteine-rich repeats in the extracellular domain. In parallel the tumor necrosis factor ligand super-family has been recognized by discovery of ligands for all members of the receptor superfamily. These molecules are also transmembrane (type II) glycoproteins, with the exception of lymphotoxin-alpha which is the only entirely secreted protein of the tumor necrosis factor-like proteins. Several members of the ligand superfamily, including tumor necrosis factor and CD95L also exist in a biologically active soluble form. The tumor necrosis factor ligand superfamily contains at present ten different proteins. In addition, NGFR p75 binds to a second family of proteins (neurotrophins). These nerve growth factor-like dimeric soluble molecules are basic neurotrophic factors and the five members (NGF, BDNF, NT-3, NT-4, NT-5) are not related to the tumor necrosis factor superfamily ligands. The members of the tumor necrosis factor ligand superfamily (TNF, LT-alpha, LT-beta, CD27L, CD30L, CD40L, CD95L, 4-IBB, OX40L, TRAIL) share common biological activities, but some properties are shared by only some ligands, while others are unique. The diverse biological activities triggered through tumor necrosis factor receptors have been linked to the regulation of cellular activation, including immune responses and inflammatory reactions, but also with the pathology of a series of human diseases.
|Recent progress in the tumor necrosis factor-alpha field.|
Rink, L and Kirchner, H
Int. Arch. Allergy Immunol., 111: 199-209 (1996) 1996
Tumor necrosis factor (TNF is used synonymously with TNF-alpha) is a pleiotropic cytokine. The effects of TNF were first described in the last century, but the soluble factor was discovered in 1975. Now TNF is one of the most important cytokines investigated by scientists from different fields. This short review briefly summarizes the state of the art and adds the news of the last 2 years. The progress in the field of TNF is focussed on gene organization and transcription, gene polymorphism, biochemistry of TNF, TNF receptors and the biological role of TNF in autoimmune diseases, infectious diseases, transplantation, cancer and TNF functions on endothelial cells.
|Inhibitory effects of sulfasalazine and its metabolites on histamine release and TNF-alpha production by mast cells|
Bissonnette, E. Y., et al
J Immunol, 156:218-23 (1996) 1996
|WEHI 164 subclone 13 assay for TNF: sensitivity, specificity, and reliability.|
Eskandari, M K, et al.
Immunol. Invest., 19: 69-79 (1990) 1990
Tumor necrosis factor alpha (TNF) is a peptide monokine involved in a number of immune reactions. To further understand the role of TNF in disease states it is critical to have an inexpensive, yet sensitive and specific assay. Additionally, the effects of prostaglandin E2 (PGE2), dexamethasone (dex), and cyclosporine A (CsA) on TNF gene expression have been studied, although little is known of the effects these compounds have on TNF containing samples. The aim of this study is to determine the sensitivity and specificity of a highly sensitive cell line to the actions of TNF, and to elucidate parameters which affect the stability of TNF in biological fluids. Dex and PGE2 at concentrations of 10(-5), 10(-7), and 10(-9) M, were shown not to effect the WEHI assay, and neither did CsA (10 ng/ml-1 ug/ml). The cells were not lysed by recombinant murine IL-1 alpha or beta, human recombinant IL-1 alpha or beta, human recombinant IL-2 or human recombinant IL-6 at concentrations ranging from 0.02 pg/ml to 1.0 ug/ml, or murine gamma-IFN from 100 pg/ml to 10 ng/ml. TNF containing samples with 1%-10% fetal calf serum maintained their cytolytic activity even after three freeze-thaw cycles. Serum samples did not lose any cytolytic activity with up to 11 cycles of freezing and thawing whereas, tissue culture media, containing TNF, lost significant activity with freeze-thawing. The WEHI assay has successfully detected cytolytic activity from lipopolysaccharide stimulated specimens from a number of different species. These data show the utility of this highly sensitive and specific assay. Furthermore, the WEHI assay showed a high degree of reproducibility in repeated assays.
|Human tumor necrosis factor. Production, purification, and characterization.|
Aggarwal, B B, et al.
J. Biol. Chem., 260: 2345-54 (1985) 1985