01-164 TNFα Protein, recombinant

01-164
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSource
      CULT Human Recombinant human TNFalpha, expressed in E. coli
      Description
      Catalogue Number01-164
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionTNFα Protein, recombinant
      OverviewMW:17kDa
      References
      Product Information
      PresentationLypholized from 3mM Tris, pH 8.0.
      Quality LevelMQ100
      Applications
      ApplicationThe recombinant TNFα Protein is available in a 10 µg format.
      Key Applications
      • Cell Culture
      Biological Information
      SourceRecombinant human TNFalpha, expressed in E. coli
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
      Gene Symbol
      • TNF
      • TNFSF2
      • Cachectin
      • cachectin
      • TNF-alpha
      • DIF
      • OTTHUMP00000037669
      • TNFA
      • TNF-a
      Purification MethodSterilized through a 0.2 micron membrane filter
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
      SIZE: 233 amino acids; 25644 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
      SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
      Stem Cell Type
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in a biological activity assay.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TNFα Protein, recombinant Certificates of Analysis

      TitleLot Number
      TNF a (Tumor Necrosis Factor a) (recombinant protein expressed in E. coli)2928459
      TNF a (Tumor Necrosis Factor a) (recombinant protein expressed in E. coli)) - 20645062064506
      TNF a (Tumor Necrosis Factor a) (recombinant protein expressed in E. coli)) - 22731442273144
      TNF a (Tumor Necrosis Factor a) (recombinant protein expressed in E. coli)) - DAM1548252DAM1548252
      TNF a (Tumor Necrosis Factor a) (recombinant protein expressed in E. coli)) - DAM1754419DAM1754419
      TNF a (Tumor Necrosis Factor a) - 20448112044811
      TNF a (Tumor Necrosis Factor a) - DAM1601816 - DAM1601816DAM1601816
      TNF a (Tumor Necrosis Factor a) - DAM1734742DAM1734742
      TNF a (Tumor Necrosis Factor a) - JBC1868992JBC1868992
      TNF α (Tumor Necrosis Factor α) (recombinant protein expressed in E. coli)) - 23970372397037

      References

      Reference overviewApplicationPub Med ID
      Interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor alpha (TNF alpha) regulate insulin-like growth factor binding protein-1 (IGFBP-1) levels and mRNA abundance in vivo and in vitro
      Benbassat, C. A., et al
      Horm Metab Res, 31:209-15 (1999)  1999

      Immunoprecipitation10226804 10226804
      Tumor necrosis factor alpha (TNFalpha) promotes growth of virulent Mycobacterium tuberculosis in human monocytes iron-mediated growth suppression is correlated with decreased release of TNFalpha from iron-treated infected monocytes
      Byrd, T. F.
      J Clin Invest, 99:2518-29 (1997)  1997

      Cell Culture9153296 9153296
      Co-regulation and physical association of the 67-kDa monomeric laminin receptor and the alpha6beta4 integrin
      Ardini, E., et al
      J Biol Chem, 272:2342-5 (1997)  1997

      Cell Culture8999943 8999943
      Molecular, structural, and biological characteristics of the tumor necrosis factor ligand superfamily.
      Gruss, H J
      Int. J. Clin. Lab. Res., 26: 143-59 (1996)  1996

      Show Abstract
      8905447 8905447
      Recent progress in the tumor necrosis factor-alpha field.
      Rink, L and Kirchner, H
      Int. Arch. Allergy Immunol., 111: 199-209 (1996)  1996

      Show Abstract
      8917114 8917114
      Inhibitory effects of sulfasalazine and its metabolites on histamine release and TNF-alpha production by mast cells
      Bissonnette, E. Y., et al
      J Immunol, 156:218-23 (1996)  1996

      8598465 8598465
      WEHI 164 subclone 13 assay for TNF: sensitivity, specificity, and reliability.
      Eskandari, M K, et al.
      Immunol. Invest., 19: 69-79 (1990)  1990

      Show Abstract
      2110931 2110931
      Human tumor necrosis factor. Production, purification, and characterization.
      Aggarwal, B B, et al.
      J. Biol. Chem., 260: 2345-54 (1985)  1985

      3871770 3871770

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors