PF019 TIMP-1, Human, Recombinant, CHO Cells

PF019
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PF019-3UG
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      Plastic ampoule 3 μg
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      Description
      OverviewRecombinant, human TIMP-1 expressed in CHO cells. Migrates as a 28 kDa protein under reducing conditions on SDS-PAGE.
      Catalogue NumberPF019
      Brand Family Calbiochem®
      SynonymsTissue Inhibitor of Metalloproteinase-1
      References
      ReferencesFreudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250.
      Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
      Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341.
      Docherty, A.J.P. et al. 1985. Nature 318, 66.
      Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
      Product Information
      ATP CompetitiveN
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLyophilized
      FormulationLyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.
      PreservativeNone
      ReversibleN
      Quality LevelMQ100
      Applications
      Application ReferencesInhibition Studies Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
      Application NotesImmunoblotting Standard (20 µl/lane)
      MMP Inhibition (assay dependent, see comments)
      Application CommentsTIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).
      Biological Information
      Biological activityIC₅₀ against native human MMP-1 is ~1-5 nM.
      Primary TargetHuman MMP 1
      Primary Target IC<sub>50</sub>1-5 nM against human MMP 1
      Purity≥95% by SDS PAGE (prior to lyophilization)
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TIMP-1, Human, Recombinant, CHO Cells SDS

      Title

      Safety Data Sheet (SDS) 

      TIMP-1, Human, Recombinant, CHO Cells Certificates of Analysis

      TitleLot Number
      PF019

      References

      Reference overview
      Freudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250.
      Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
      Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341.
      Docherty, A.J.P. et al. 1985. Nature 318, 66.
      Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.

      Citations

      Title
    • William J. Lane, et al. (2000) Stromal-derived factor 1-induced megakaryocyte migration and platelet production is dependent on matrix metalloproteinases. Blood 96, 4152-4159.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-September-2007 JSW
      SynonymsTissue Inhibitor of Metalloproteinase-1
      ApplicationImmunoblotting Standard (20 µl/lane)
      MMP Inhibition (assay dependent, see comments)
      DescriptionRecombinant, human TIMP-1 expressed in CHO cells. Migrates as a ~28 kDa protein by SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
      BackgroundThe activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
      FormLyophilized
      FormulationLyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 µg BSA, pH 7.3.
      Recommended reaction conditions
      Immunoblotting Standard Useful as an immunoblotting standard; use at 20 µl/lane for most applications. MMP Inhibition Assays TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).
      Purity≥95% by SDS PAGE (prior to lyophilization)
      Biological activityIC₅₀ against native human MMP-1 is ~1-5 nM.
      SolubilityReconstitute with PBS to the desired concentration and mix gently until completely dissolved.
      PreservativeNone
      CommentsTIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC50 of 1-5 nM (see application references).
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesFreudenstein, J. et al. 1990. Biochem. Biophys. Res. Comm. 171, 250.
      Liotta, L. A., and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 99.
      Kodama, S. et al. 1987. Collagen Rel. Res. 7, 341.
      Docherty, A.J.P. et al. 1985. Nature 318, 66.
      Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.
      Citation
    • William J. Lane, et al. (2000) Stromal-derived factor 1-induced megakaryocyte migration and platelet production is dependent on matrix metalloproteinases. Blood 96, 4152-4159.
    • Application referencesInhibition Studies Nagai, Y., et al. 1984. Jpn. J. Inflam. 4, 123.