SCR508 TAT-CRE Recombinase

SCR508
1500 units  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCR508
      DescriptionTAT-CRE Recombinase
      Alternate Names
      • Integrase
      • Recombinase Cre
      Background InformationCre Recombinase is an enzyme from bacteriophage P1 that catalyzes the site-specific recombination between two DNA recognition sites termed loxP sites. The LoxP recognition site consists of two 13 bp inverted repeats flanking a 8 bp spacer region. Because the Cre gene and loxP sites are not native to the genomes of most species, LoxP sites can be engineered and introduced into target cells and thus used as a means to precisely control the expression of genes in vitro (i.e. cultured cells) and in vivo (i.e. animal models).

      • If LoxP sites are located on different chromosomes, Cre recombinase will mediate a chromosomal translocation.
      • If LoxP sites are oriented in the opposite direction, Cre recombinase will mediate an inversion of the floxed segment.
      • If LoxP sites are oriented in the same direction, Cre recombinase will mediate a deletion of the floxed segment.

      In this way, placement of the LoxP sites allows genes to be activated, repressed or exchanged for other genes.

      EMD Millipore’s TAT-CRE Recombinase is a recombinant cell-permeant fusion protein consisting of a basic protein translocation peptide derived from HIV-TAT (TAT), a nuclear localization sequence (NLS), the Cre protein and an N-terminal histidine tag (H6) for efficient purification of the protein from E. coli.

      EMD Millipore’s TAT-CRE Recombinase has been shown to effectively excise STEMCCA viral transgenes from both Human and Mouse IPS cells.
      References
      Product Information
      PresentationRecombinant protein is supplied in buffer containing 50% glycerol (v/v) 500 mM NaCl and 20 mM HEPES at pH 7.4
      ActivityA standard of 100 Units is defined as the amount of TAT-CRE (ug) in 1.0 mL of tissue culture medium that is required to induce 50% GFP expression in a HEK293T loxp reporter cell line assay.
      Quality LevelMQ100
      Applications
      ApplicationTAT-CRE Recombinase is a recombinant cell-permeant fusion cre-recombinase protein consisting of TAT sequence, a nuclear localization sequence (NLS) and it is known to catalyze the site specific recombination event between two loxP DNA sites.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Application NotesEMD Millipore has developed a cell-permeant TAT-CRE Recombinase fusion protein which can be directly delivered to mammalian cells and results in high recombination efficiencies (75 – 100% in mouse and ~ 60% in human cells). TAT-CRE readily translocates to mammalian cells and can catalyze highly efficient recombination. The dose and timing of Cre exposure can be precisely controlled thus allowing for the careful titration of Cre activity.
      Biological Information
      HostE. coli
      Physicochemical Information
      ActivityA standard of 100 Units is defined as the amount of TAT-CRE (ug) in 1.0 mL of tissue culture medium that is required to induce 50% GFP expression in a HEK293T loxp reporter cell line assay.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of TAT-CRE Recombinase protein is rigorously quality control tested for the following parameters:
      • Purity: single band around 41 kDa with greater than 70% protein purity on an SDS-PAGE gel
      • Functional activity: mediates recombination of LoxP-modified alleles in a HEK293T- Cre reporter cell line
      • Endotoxin levels: less than 1 EU/ug protein
      • Mycoplasma negative
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 3 months from date of receipt when stored at -20°C or -80°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C. Before use, dilute TAT-CRE to the appropriate concentration with culture medium and filter through a 0.2um low protein binding syringe filter (Millipore Cat. No. SLGV 033RS or SLGV013 SL).
      Packaging Information
      Material Size1500 units
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TAT-CRE Recombinase SDS

      Title

      Safety Data Sheet (SDS) 

      TAT-CRE Recombinase Certificates of Analysis

      TitleLot Number
      TAT-CRE Recombinase2452169
      TAT-CRE Recombinase - 23291882329188
      TAT-CRE Recombinase - 23588622358862
      TAT-CRE Recombinase - RD1402001RD1402001
      TAT-CRE Recombinase - 25204942520494
      TAT-CRE Recombinase - 32307083230708
      TAT-CRE Recombinase - 32513543251354
      TAT-CRE Recombinase - 32902893290289
      TAT-CRE Recombinase - 33187893318789
      TAT-CRE Recombinase - 34069723406972

      References

      Reference overviewPub Med ID
      Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells.
      Kadari, Asifiqbal, et al.
      Stem Cell Res Ther, 5: 47 (2014)  2014

      Show Abstract
      24713299 24713299
      Stem cell engineering using transducible Cre recombinase.
      Nolden, Lars, et al.
      Methods Mol. Med., 140: 17-32 (2007)  2007

      Show Abstract
      18085201 18085201
      Stage-specific conditional mutagenesis in mouse embryonic stem cell-derived neural cells and postmitotic neurons by direct delivery of biologically active Cre recombinase.
      Haupt, Simone, et al.
      Stem Cells, 25: 181-8 (2007)  2007

      Show Abstract
      16960133 16960133
      Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase.
      Nolden, Lars, et al.
      Nat. Methods, 3: 461-7 (2006)  2006

      Show Abstract
      16721380 16721380

      Brochure

      Title
      Simplifying the iPSC Workflow

      User Guides

      Title
      TAT-CRE Recombinase